Difference between revisions of "Assignment 1 Overview: Transillumination microscopy"
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Make sure to include answers to all the following questions: | Make sure to include answers to all the following questions: | ||
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# Answers to the pre-lab questions [[Assignment 1, Part 1: Pre-lab questions|listed at the bottom of the Part 1 page]]. | # Answers to the pre-lab questions [[Assignment 1, Part 1: Pre-lab questions|listed at the bottom of the Part 1 page]]. | ||
− | Part 2 | + | Part 2: |
# Turn in your measured focal lengths for each lens A through D. | # Turn in your measured focal lengths for each lens A through D. | ||
# Make a block diagram of the apparatus (a sketch is fine). You do not need to detail the mechanical construction, but be sure to include any optical elements (light sources, lenses, cameras), as well as the object being measured. Label each component as well as the distances you will be varying (<math>S_i</math> and <math>S_o</math>). Do not label distances that are irrelevant. | # Make a block diagram of the apparatus (a sketch is fine). You do not need to detail the mechanical construction, but be sure to include any optical elements (light sources, lenses, cameras), as well as the object being measured. Label each component as well as the distances you will be varying (<math>S_i</math> and <math>S_o</math>). Do not label distances that are irrelevant. | ||
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# Describe how noise varies as a function of light intensity. (Notice that the axes of this plot are in log scale. [[Understanding log plots|Click here]] if you'd like a refresher how to interpret log-log plots.) Did the plot look the way you expected? | # Describe how noise varies as a function of light intensity. (Notice that the axes of this plot are in log scale. [[Understanding log plots|Click here]] if you'd like a refresher how to interpret log-log plots.) Did the plot look the way you expected? | ||
− | Parts 3 and 4 | + | Parts 3 and 4: |
# Display an example image of the ruler at each magnification, and | # Display an example image of the ruler at each magnification, and | ||
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# Discuss how the measured bead sizes compared to the nominal size. | # Discuss how the measured bead sizes compared to the nominal size. | ||
# In one or two sentences, explain how you chose the number of samples to measure. | # In one or two sentences, explain how you chose the number of samples to measure. | ||
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Revision as of 20:32, 5 September 2018
Introduction
Over the next few weeks, you will build an optical microscope using lenses, mirrors, filters, optical mounts, CCD cameras, lasers, and other components in the lab. The work is divided into 5 assignments. Each assignment requires some problem solving, some lab work, some analysis, lots of clear thinking, and an individually written answer sheet turned in on Stellar. All of the items you are expected to turn in are indicated by a pencil symbol in the lab manual.
This symbol means that you have to turn something in. |
Background reading and resources
You will work with log-log plots in this assignment and future ones. These seem to confuse everybody. Read this page to remind yourself how log-log plots work.
Several microscope manufacturers maintain educational websites, including Nikon's MicroscopyU, Olympus' Microscopy Primer, and the Zeiss online microscopy campus. The content on these sites ranges from basic concepts like Snell's law and Resolution to advanced techniques like super resolution imaging.
Assignment details
This assignment has 4 parts:
- Part 1: Learn about optics and answer a few questions before you start your lab work;
- Part 2: Some warm-up lab exercises;
- Part 3: You will build a microscope; and finally you will
- Part 4: Measure its magnification and the size of some small beads.
You will add fluorescence capability in the next part of the lab.
Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment1.pdf. Here is a checklist of all things you have to turn in:
Make sure to include answers to all the following questions: Part 1:
Part 2:
Parts 3 and 4:
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- Overview
- Part 1: Pre-lab questions
- Part 2: Optics bootcamp
- Part 3: Build a microscope
- Part 4: Measure stuff
Back to 20.309 Main Page
References