Difference between revisions of "20.109:Module 4"
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==Module 4== | ==Module 4== | ||
| − | '''Instructors:''' [ | + | '''Instructors:''' [http://openwetware.org/wiki/Angela_Belcher ] and [http://openwetware.org/wiki/Natalie_Kuldell ] |
| − | '''TA:''' [ | + | '''TA:''' [http://openwetware.org/wiki/Chandni_Valiathan ] |
| − | In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then | + | In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we ll elucidate some amino acid requirements for the yeast/metal interaction. |
[[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']] | [[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']] | ||
| − | [[ | + | [[20.109(S07):Bio-material engineering/Screening library | Module 4 Day 1: Screening library]]<br> |
| − | [[ | + | [[20.109(S07): Bio-material engineering/Optimizing panning | Module 4 Day 2: Optimizing panning]]<br> |
| − | [[ | + | [[20.109(S07): Bio-material engineering/Rescreening gold binders | Module 4 Day 3: Rescreening gold binders]]<br> |
| − | [[ | + | [[20.109(S07): Bio-material engineering/PCR of gold binding candidates | Module 4 Day 4: PCR of gold binding candidates]]<br> |
| − | [ | + | [http://openwetware.org/wiki/20.109_(S07):_Research_proposal,_module_4 | Module 4 Day 5: Student presentations]<br> |
| − | [[ | + | [[20.109(S07): Bio-material engineering/Sequence analysis | Module 4 Day 6: Sequence analysis]]<br> |
| + | [http://openwetware.org/wiki/20.109(S07):_Biomaterial_engineering,_TA_notes | TA notes Module 4] | ||
Latest revision as of 15:31, 15 June 2015
Module 4
TA: [3]
In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we���ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we���ll elucidate some amino acid requirements for the yeast/metal interaction.
Module 4 Day 1: Screening library
Module 4 Day 2: Optimizing panning
Module 4 Day 3: Rescreening gold binders
Module 4 Day 4: PCR of gold binding candidates
| Module 4 Day 5: Student presentations
Module 4 Day 6: Sequence analysis
