20.109(S22):Module 2
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Contents
Module 2: metabolic engineering
Research goal: XX
Lab links: day to day
M2D1: Complete in-silico cloning of pdCas9 expression plasmid
M2D2: Design gRNA sequence for CRIPSRi system
M2D3: Clone psgRNA expression plasmid
M2D4: Confirm gRNA sequence in psgRNA expression plasmid
M2D5: Prepare for induction of CRISPRi system
M2D6: Complete CRISPRi experiment and measure fermentation products
M2D7: Examine features in gRNA-targeted genomic sequences
M2D8: Organize figures and outline text for Research article
Major assignments
Journal Club presentation
Research article
References
- CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Methods. (2013) 8: 2180-2196.
- Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Frontiers in Bioengineering and Biotechnology. (2014) 2:1-12.
