Difference between revisions of "20.109(S22):Class data"

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Revision as of 20:42, 8 February 2022

20.109(S22): Laboratory Fundamentals of Biological Engineering

Sp17 20.109 M1D7 chemical structure features.png

Spring 2022 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Metabolic engineering        M3: Project design       


Module 1: Drug discovery

small molecule selections

T/R

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID)
TR Red
TR Orange
TR Yellow
TR Green 69269200 83079118
TR Blue
TR Teal
TR Pink 83079118 69269200
TR Purple
TR Grey 69269200 83079118
TR White

W/F

Team small molecule #1 (enter compound ID) small molecule #2 (enter compound ID)
WF Red
WF Orange
WF Yellow
WF Green
WF Blue
WF Teal
WF Pink
WF Purple
WF Grey
WF White

Module 2: Metabolic engineering

sgRNA_target sequences

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Ethanol / Acetate Assay Results
TR Red
TR Orange
TR Yellow
TR Green
TR Blue
TR Teal
TR Pink
TR Purple
TR Grey
TR White

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target coding or non-coding strand Colorimetric Assay Results
WF Red
WF Orange
WF Yellow
WF Green
WF Blue
WF Teal
WF Pink
WF Purple
WF Grey
WF White