Difference between revisions of "20.109(S18): Spring 2018 schedule"

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| T/W Apr 18/19
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| T/W Apr 17/18
 
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| <font color = #999999>Patriots' day holiday</font color>
 
| <font color = #999999>Patriots' day holiday</font color>
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| 3
 
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| R/F Apr 20/21
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| R/F Apr 19/20
| [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109_M3L1.pptx.pdf | M3L1]] 
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| [http://be.mit.edu/directory/angela-belcher AB]<br>
| [[20.109(S17):Growth of phage materials (Day1) | Grow phage active material]]<br>[[Media:Sp17_M3D1_170420_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D1_LMM_1.pdf| Prelab W/F]]
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| [[20.109(S18):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br>
| [[20.109(S17):Homework#Due_M3D1| Homework due]] <br> [[20.109(S17): Research article| Research article due]] April 22 at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] Sun, Apr 23 at 10 pm
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| [[20.109(S18):Homework#Due_M3D1| Homework due]] <br> [[20.109(S18): Research article| Research article due]] DATE at 10 pm <br> [http://be20109s17.blogspot.com/ Blog post due] DATE at 10 pm
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
| T/W Apr 25/26
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| T/W Apr 24/25
| [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:20109 M3L2.pptx | M3L2]] 
+
| [http://be.mit.edu/directory/angela-belcher AB]<br>
| [[20.109(S17):Purify active materials (Day2) | Purify phage active material]]<br>[[Media:Sp17_M3D2_170425_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D2_LMM_1.pdf| Prelab W/F]]
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| [[20.109(S18):Purify phage-based active (cathode) material (Day2) | Purify phage-based active (cathode) material]]<br>
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D2| Homework due]]
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| <font color=#FE2E2E>'''Laboratory quiz'''</font color> <br> [[20.109(S18):Homework#Due_M3D2| Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 3
 
| 3
| R/F Apr 27/28
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| R/F Apr 26/27
 
|[http://be.mit.edu/directory/angela-belcher AB]<br>
 
|[http://be.mit.edu/directory/angela-belcher AB]<br>
| [[20.109(S17):Cathode construction (Day3)| Construct cathode with phage active material]]<br>[[Media:Sp17_M3D3_170427_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D3_LMM_1.pdf| Prelab W/F]]
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| [[20.109(S18):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br>
| [[20.109(S17):Homework#Due_M3D3| Homework due]]
+
| [[20.109(S18):Homework#Due_M3D3| Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
|  
 
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| T/W May 2/3
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| T/W May 1/2
 
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| <font color = red>Lecture, but no laboratory</font color>
 
| <font color = red>Lecture, but no laboratory</font color>
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| 3
 
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| 4
| R/F May 4/5
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| R/F May 3/4
 
| [http://be.mit.edu/directory/angela-belcher AB]
 
| [http://be.mit.edu/directory/angela-belcher AB]
| [[20.109(S17):TEM (Day4)| Visualize phage nanowires using TEM]]<br>[[Media:Sp17_M3D4_170504_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D4_LMM_1.pdf| Prelab W/F]]
+
| [[20.109(S18):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using TEM]]<br>
| [[20.109(S17):Homework#Due_M3D4| Homework due]]
+
| [[20.109(S18):Homework#Due_M3D4| Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
| T/W May 9/10
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| T/W May 8/9
 
| [http://be.mit.edu/directory/angela-belcher AB]
 
| [http://be.mit.edu/directory/angela-belcher AB]
| [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]]<br>[[Media:Sp17_M3D5_170509_marked.pdf| Prelab T/R]]<br>[[Media:Sp17_M3D5_LMM.pdf| Prelab W/F]]
+
| [[20.109(S18):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br>
| <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M3D5| Homework due]]
+
| <font color=#FE2E2E>'''Laboratory quiz'''</font color> <br> [[20.109(S18):Homework#Due_M3D5| Homework due]]
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
| R/F May 11/12
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| R/F May 10/11
|<font color = red>no lecture</font color>
+
| [[20.109(S17):Research proposal presentation| Research proposal presentations]]
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| [http://be20109s17.blogspot.com/ Blog post due] Thu/Fri, May 11/12 at 10 pm
+
|--
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|
+
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| T/W May 16/17
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|
 
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| Open office hours
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| [[20.109(S18):Research proposal presentations | Research proposal presentations]]
| [[20.109(S17): Biomaterials engineering mini-report| Mini report due]] Tue/Wed, May 16/17 at 10 pm  
+
| [http://be20109s17.blogspot.com/ Blog post due] DATE at 10 pm
 
|--
 
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| R May 18
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| T May 15
 
|  
 
|  
 
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color>
 
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color>

Revision as of 15:07, 4 January 2018

20.109(S18): Laboratory Fundamentals of Biological Engineering

Sp18 banner image v2.png

Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              

Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 6/7 NLL
Orientation
1 1 R/F Feb 8/9 AK
In silico cloning and confirmation digest
Orientation quiz
Homework due
1 2 T/W Feb 13/14 AK
Complete small microarry analysis and induce protein expression
Homework due
1 3 R/F Feb 15/16 AK
Purify protein
Laboratory quiz
Homework due
T/W Feb 20/21 President's day holiday
1 4 R/F Feb 22/23 AK
Assess protein purity and concentration
Homework due
1 5 T/W Feb 27/28 AK
SECONDARY ASSAY D1
Laboratory quiz
Homework due
1 6 R/F Mar 1/2 AK
SECONDARY ASSAY D2
Homework due
1 7 T/W Mar 6/7 AK
Complete data analysis
Laboratory quiz
Homework due
2 1 R/F Mar 8/9 NLL
Practice tissue culture techniques and seed cells for RNA purification
Homework due
2 2 T/W Mar 13/14 NLL
Induce DNA damage and apply drug treatments for RNA purification
Laboratory quiz
Homework due
Data Summary draft due DATE at 10 pm
2 3 R/F Mar 15/16 EF
Purify RNA and practice RNA-seq data analysis methods
Homework due
Mini presentation due DATE at 10 pm
2 4 T/W Mar 20/21 EF
Analyze RNA-seq data and prepare for quantitative PCR experiment
Laboratory quiz
Homework due
2 5 R/F Mar 22/23 EF
ANALYZE RNA SEQ DATA Homework due
T/W Mar 27/28 Spring vacation Data Summary revision due DATE at 10 pm
R/F Mar 29/30 Spring vacation
2 6 T/W Apr 3/4 Comm Lab
Journal club I
Blog post due DATE at 10 pm
2 7 R/F Apr 5/6 NLL
Journal club II
2 8 T/W Apr 10/11 EF
Induce DNA damage and apply drug treatments for cell viability and RNA_SEQ DATA ANALYSIS Homework due
2 9 R/F Apr 12/13 EF
Complete cell viability assay
Laboratory quiz
Homework due
Blog post due DATE at 10 pm
T/W Apr 17/18 Patriots' day holiday
3 1 R/F Apr 19/20 AB
Grow phage-based active (cathode) material
Homework due
Research article due DATE at 10 pm
Blog post due DATE at 10 pm
3 2 T/W Apr 24/25 AB
Purify phage-based active (cathode) material
Laboratory quiz
Homework due
3 3 R/F Apr 26/27 AB
Construct cathode with phage-based active material
Homework due
3 T/W May 1/2 Lecture, but no laboratory
3 4 R/F May 3/4 AB Visualize phage nanowires using TEM
Homework due
3 5 T/W May 8/9 AB Assemble and test batteries
Laboratory quiz
Homework due
R/F May 10/11 Research proposal presentations Blog post due DATE at 10 pm
T May 15 Feedback and celebratory lunch