20.109(S14):Begin Western protein analysis (Day2)

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20.109(S14): Laboratory Fundamentals of Biological Engineering

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Home        Schedule Spring 2014        Assignments       
Module 1        Module 2        Module 3              

Introduction

Topic 1: Reiterate purpose of WB, now some details about cell lines

Topic 2: Basis of WB, including background on antibodies

Protocols

Part 1: Prepare cell lysates

Part 2: Measure protein content

Part 3: Separate proteins by SDS-PAGE

Part 4: Transfer proteins to membrane

For next time

Reagent list

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Navigation Links

Next Day: Choose system conditions and paper discussion

Previous Day: Introduction to cell strains and plating
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