Difference between revisions of "20.109(S13):Initiating transcript and protein assays (Day5)"
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==Introduction== | ==Introduction== | ||
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There are several ways to assess protein concentration, either quantitatively or as present above some threshold value. In the second module, you used a colorimetric Coomassie-based assay to measure the concentration of protein expressed by your bacteria. Because you were purifying a His-tagged protein from bacteria induced primarily to express said protein, you could assume that the protein concentration that you measured was primarily inverse pericam. In contrast, today you are trying to measure the concentration of a specific protein that is only one among many in a complex mixture. | There are several ways to assess protein concentration, either quantitatively or as present above some threshold value. In the second module, you used a colorimetric Coomassie-based assay to measure the concentration of protein expressed by your bacteria. Because you were purifying a His-tagged protein from bacteria induced primarily to express said protein, you could assume that the protein concentration that you measured was primarily inverse pericam. In contrast, today you are trying to measure the concentration of a specific protein that is only one among many in a complex mixture. | ||
− | A great way to identify a specific protein from a complex mixture is to exploit antibodies | + | A great way to identify a specific protein from a complex mixture is to exploit antibodies |