Difference between revisions of "20.109(S10): Spring 2010 schedule"

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| Thur Feb 4
 
| Thur Feb 4
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
 
| [[20.109(S10):Amplify aptamer-encoding DNA (Day1)| Amplify aptamer-encoding DNA]]
 
|*[[Media:20109_S09-M1Lec1_Niles.pdf | M1D1 Lecture]]<br> *[[Media:S10_M1D1-ANS.pdf | M1D1 Pre-Lab]]
 
|*[[Media:20109_S09-M1Lec1_Niles.pdf | M1D1 Lecture]]<br> *[[Media:S10_M1D1-ANS.pdf | M1D1 Pre-Lab]]
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| Tue Feb 9
 
| Tue Feb 9
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
 
| [[20.109(S10):Purify aptamer-encoding DNA (Day2)| Purify aptamer-encoding DNA]]
 
|*[[Media:20109_S10-M1Lec2_Niles.pdf | M1D2 Lecture]]<br> *[[Media:S10_M1D2-ANS.pdf | M1D2 Pre-Lab]]
 
|*[[Media:20109_S10-M1Lec2_Niles.pdf | M1D2 Lecture]]<br> *[[Media:S10_M1D2-ANS.pdf | M1D2 Pre-Lab]]
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| Thur Feb 11
 
| Thur Feb 11
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
 
| [[20.109(S10):Prepare RNA by IVT (Day3)| Prepare RNA by IVT]]
 
|*[[Media:20109_S10-M1Lec3_Niles.pdf | M1D3 Lecture]]<br> *[[Media:S10_M1D3-ANS.pdf | M1D3 Pre-Lab]]
 
|*[[Media:20109_S10-M1Lec3_Niles.pdf | M1D3 Lecture]]<br> *[[Media:S10_M1D3-ANS.pdf | M1D3 Pre-Lab]]
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| Thur Feb 18
 
| Thur Feb 18
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
 
| [[20.109(S10):Purify RNA and run affinity column (Day4)| Purify RNA and run affinity column]]
 
|*[[Media:20109_S10-M1Lec4_Niles.pdf | M1D4 Lecture]]<br> *[[Media:S10_M1D4-ANS.pdf | M1D4 Pre-Lab]]
 
|*[[Media:20109_S10-M1Lec4_Niles.pdf | M1D4 Lecture]]<br> *[[Media:S10_M1D4-ANS.pdf | M1D4 Pre-Lab]]
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| Tue Feb 23
 
| Tue Feb 23
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
 
| [[20.109(S10):RNA to DNA by RT-PCR (Day5)| RNA to DNA by RT-PCR]]
 
|*[[Media:20109_S10-M1Lec5_Niles.pdf | M1D5 Lecture]]<br> *[[Media:S10_M1D5-ANS.pdf | M1D5 Pre-Lab]]
 
|*[[Media:20109_S10-M1Lec5_Niles.pdf | M1D5 Lecture]]<br> *[[Media:S10_M1D5-ANS.pdf | M1D5 Pre-Lab]]
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| Thur Feb 25
 
| Thur Feb 25
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
 
| [[20.109(S10):Post-selection IVT and journal club (Day6)| Post-selection IVT and journal club]]
 
| *[[Media:20109_S10-M1Lec6_Niles.pdf | M1D6 Lecture]]<br> *[[Media:S10_M1D6-ANS.pdf | M1D6 Pre-Lab]]
 
| *[[Media:20109_S10-M1Lec6_Niles.pdf | M1D6 Lecture]]<br> *[[Media:S10_M1D6-ANS.pdf | M1D6 Pre-Lab]]
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| Tue Mar 2
 
| Tue Mar 2
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]]
 
| [[20.109(S10):Aptamer binding assay (Day7)| Aptamer binding assay]]
|
+
|*[[Media:20109_S10-M1Lec7_Niles.pdf | M1D7 Lecture]]<br> *[[Media:S10_M1D7-ANS.pdf | M1D7 Pre-Lab]]
 
|--
 
|--
 
| 1
 
| 1
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| Thur Mar 4
 
| Thur Mar 4
 
| [http://web.mit.edu/be/people/niles.htm JN]
 
| [http://web.mit.edu/be/people/niles.htm JN]
| [[User:Christina_M_Birch| CB]]
+
| [http://openwetware.org/wiki/User:Christina_M_Birch | CB]
 
| [[20.109(S10):Journal club (Day8)| Journal club]]  
 
| [[20.109(S10):Journal club (Day8)| Journal club]]  
|
+
|*[[Media:20109_S10-M1Lec8_Niles.pdf | M1D8 Lecture]]
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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| Tue Mar 9
 
| Tue Mar 9
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Start-up protein engineering (Day1)| Start-up protein engineering]]
 
| [[20.109(S10):Start-up protein engineering (Day1)| Start-up protein engineering]]
| <font color = CC0000><b>Module 1 Draft Due</b></font color><br>
+
| <font color = CC0000><b>Module 1 Draft Due</b></font color><br>*[[Media:20109_S10-M2Lec1_Jasanoff.pdf | M2D1 Lecture]]<br> *[[Media:S10_M2D1-ANS.pdf | M2D1 Pre-Lab]]
 
|--
 
|--
 
| 2
 
| 2
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| Thur Mar 11
 
| Thur Mar 11
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
 
| [[20.109(S10):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
|
+
|*[[Media:20109_S10-M2Lec2_Jasanoff.pdf | M2D2 Lecture]]<br> *[[Media:S10_M2D2-ANS.pdf | M2D2 Pre-Lab]]
 
|--
 
|--
 
| 2
 
| 2
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| Tue Mar 16
 
| Tue Mar 16
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
 
| [[20.109(S10):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
|
+
|*[[Media:20109_S10-M2Lec3_Jasanoff.pdf | M2D3 Lecture]]<br> *[[Media:S10_M2D3-ANS.pdf | M2D3 Pre-Lab]]
 
|--
 
|--
 
| 2
 
| 2
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| Thur Mar 18
 
| Thur Mar 18
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Prepare expression system (Day4)| Prepare expression system]]
 
| [[20.109(S10):Prepare expression system (Day4)| Prepare expression system]]
|
+
|*[[Media:20109_S10-M2Lec4_Jasanoff.pdf | M2D4 Lecture]]<br> *[[Media:S10_M2D4-ANS.pdf | M2D4 Pre-Lab]]
 
|--
 
|--
 
|
 
|
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| Tue Mar 30
 
| Tue Mar 30
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
 
| [[20.109(S10):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
|  
+
| *[[Media:20109_S10-M2Lec5_Jasanoff.pdf | M2D5 Lecture]]<br> *[[Media:S10_M2D5-ANS.pdf | M2D5 Pre-Lab]]
 
|--
 
|--
 
| 2
 
| 2
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| Thur Apr 1
 
| Thur Apr 1
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Characterize protein expression (Day6)| Characterize protein expression]]
 
| [[20.109(S10):Characterize protein expression (Day6)| Characterize protein expression]]
| <font color = CC0000><b>Module 1 Final Report Due</b></font color><br>
+
| <font color = CC0000><b>Module 1 Final Report Due</b></font color><br>*[[Media:20109_S10-M2Lec6_Jasanoff.pdf | M2D6 Lecture]]<br> *[[Media:S10_M2D6-ANS.pdf | M2D6 Pre-Lab]]
 
|--
 
|--
 
| 2
 
| 2
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| Tue Apr 6
 
| Tue Apr 6
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Assay protein behavior (Day7)| Assay protein behavior]]
 
| [[20.109(S10):Assay protein behavior (Day7)| Assay protein behavior]]
|
+
|*[[Media:20109_S10-M2Lec7_Jasanoff.pdf | M2D7 Lecture]]
 
|--
 
|--
 
| 2
 
| 2
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| Thur Apr 8
 
| Thur Apr 8
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
 
| [http://web.mit.edu/be/people/jasanoff.htm AJ]
| [[User:Xiaosai Yao| XY]]
+
| [http://openwetware.org/wiki/User:Xiaosai_Yao | XY]
 
| [[20.109(S10):Data analysis (Day8)| Data analysis]]
 
| [[20.109(S10):Data analysis (Day8)| Data analysis]]
|
+
|*[[Media:20109_S10-M2Lec8_Jasanoff.pdf | M2D8 Lecture]]
 
|--
 
|--
 
| colspan="7" |  
 
| colspan="7" |  
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| 1
 
| 1
 
| Tues Apr 13
 
| Tues Apr 13
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]]
 
| [[20.109(S10):Start-up biomaterials engineering (Day1) | Start-up biomaterials engineering]]
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>
+
| <font color = CC0000><b>Module 2 Draft Due</b></font color><br>*[[Media:20109_S10-M3Lec1_Stachowiak.pdf | M3D1 Lecture]]<br>*[[Media:S10_M3D1-ANS.pdf | M3D1 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
 
| Thur Apr 15
 
| Thur Apr 15
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Initiate cell culture (Day2) | Initiate cell culture]]
 
| [[20.109(S10):Initiate cell culture (Day2) | Initiate cell culture]]
|
+
|*[[Media:20109_S10-M3Lec2_Stachowiak.pdf | M3D2 Lecture]]
 
|--
 
|--
 
|
 
|
Line 188: Line 188:
 
| 3
 
| 3
 
| Thur Apr 22
 
| Thur Apr 22
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Testing cell viability (Day3) | Testing cell viability]]
 
| [[20.109(S10):Testing cell viability (Day3) | Testing cell viability]]
|
+
|*[[Media:20109_S10-M3Lec3_Stachowiak.pdf | M3D3 Lecture]]<br>*[[Media:S10_M3D3-ANS.pdf | M3D3 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 4
 
| 4
 
| Tue Apr 27
 
| Tue Apr 27
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Preparing cells for analysis (Day4) | Preparing cells for analysis]]
 
| [[20.109(S10):Preparing cells for analysis (Day4) | Preparing cells for analysis]]
|
+
|*[[Media:20109_S10-M3Lec4_Stachowiak.pdf | M3D4 Lecture]]<br>*[[Media:S10_M3D4-ANS.pdf | M3D4 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
 
| Thur Apr 29
 
| Thur Apr 29
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Transcript-level analysis (Day5) | Transcript-level analysis]]
 
| [[20.109(S10):Transcript-level analysis (Day5) | Transcript-level analysis]]
|
+
|*[[Media:20109_S10-M3Lec5_Stachowiak.pdf | M3D5 Lecture]]<br>*[[Media:S10_M3D5-ANS.pdf | M3D5 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 6
 
| 6
 
| Tue May 4
 
| Tue May 4
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Protein-level analysis (Day6) | Protein-level analysis]]
 
| [[20.109(S10):Protein-level analysis (Day6) | Protein-level analysis]]
| <font color = CC0000><b>Module 2 Final Report Due</b></font color><br>
+
| <font color = CC0000><b>Module 2 Final Report Due</b></font color><br>*[[Media:20109_S10-M3Lec6_Stachowiak.pdf | M3D6 Lecture]]<br>*[[Media:S10_M3D6-ANS.pdf | M3D6 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 7
 
| 7
 
| Thur May 6
 
| Thur May 6
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Wrap-up analysis (Day7) | Wrap-up analysis]]
 
| [[20.109(S10):Wrap-up analysis (Day7) | Wrap-up analysis]]
| <font color = CC0000><b>Module 3 Report Due</b></font color><br>
+
| <font color = CC0000><b>Module 3 Report Due</b></font color><br>*[http://openwetware.org/images/d/d5/Mod4_presentations.pdf  Atissa's Lecture]<br>*[[Media:S10_M3D7-ANS.pdf | M3D7 Pre-Lab]]
 
|--
 
|--
 
| 3
 
| 3
 
| 8
 
| 8
 
| Tue May 11
 
| Tue May 11
| [[User:AgiStachowiak | ANS]]
+
| [http://openwetware.org/wiki/User:AgiStachowiak | ANS]
| [[User:Nicole_Casasnovas | NC]]
+
| [http://openwetware.org/wiki/User:Nicole_Casasnovas | NC]
 
| [[20.109(S10):Student presentations (Day8) | Student presentations]]
 
| [[20.109(S10):Student presentations (Day8) | Student presentations]]
 
|
 
|

Latest revision as of 19:48, 28 July 2015


20.109(S10): Laboratory Fundamentals of Biological Engineering

20.109(S09) frontpg.JPG

Home        People        Schedule Spring 2010        Assignments        Lab Basics        OWW Basics       
RNA Engineering        Protein Engineering        Cell-Biomaterial Engineering              

MODULE DAY DATE LECTURER TA LAB note
Tue Feb 2 ANS Orientation * Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
1 1 Thur Feb 4 JN CB] Amplify aptamer-encoding DNA * M1D1 Lecture
* M1D1 Pre-Lab
1 2 Tue Feb 9 JN CB] Purify aptamer-encoding DNA * M1D2 Lecture
* M1D2 Pre-Lab
1 3 Thur Feb 11 JN CB] Prepare RNA by IVT * M1D3 Lecture
* M1D3 Pre-Lab
President's Day Feb 15 No lab Feb 16 or Feb 17
1 4 Thur Feb 18 JN CB] Purify RNA and run affinity column * M1D4 Lecture
* M1D4 Pre-Lab
1 5 Tue Feb 23 JN CB] RNA to DNA by RT-PCR * M1D5 Lecture
* M1D5 Pre-Lab
1 6 Thur Feb 25 JN CB] Post-selection IVT and journal club * M1D6 Lecture
* M1D6 Pre-Lab
1 7 Tue Mar 2 JN CB] Aptamer binding assay * M1D7 Lecture
* M1D7 Pre-Lab
1 8 Thur Mar 4 JN CB] Journal club * M1D8 Lecture
2 1 Tue Mar 9 AJ XY] Start-up protein engineering Module 1 Draft Due
* M2D1 Lecture
* M2D1 Pre-Lab
2 2 Thur Mar 11 AJ XY] Site-directed mutagenesis * M2D2 Lecture
* M2D2 Pre-Lab
2 3 Tue Mar 16 AJ XY] Bacterial amplification of DNA * M2D3 Lecture
* M2D3 Pre-Lab
2 4 Thur Mar 18 AJ XY] Prepare expression system * M2D4 Lecture
* M2D4 Pre-Lab
Spring Break Mar 22-26 No lab all week
2 5 Tue Mar 30 AJ XY] Induce protein and evaluate DNA * M2D5 Lecture
* M2D5 Pre-Lab
2 6 Thur Apr 1 AJ XY] Characterize protein expression Module 1 Final Report Due
* M2D6 Lecture
* M2D6 Pre-Lab
2 7 Tue Apr 6 AJ XY] Assay protein behavior * M2D7 Lecture
2 8 Thur Apr 8 AJ XY] Data analysis * M2D8 Lecture
3 1 Tues Apr 13 ANS] NC] Start-up biomaterials engineering Module 2 Draft Due
* M3D1 Lecture
* M3D1 Pre-Lab
3 2 Thur Apr 15 ANS] NC] Initiate cell culture * M3D2 Lecture
Patriot's Day April 19-20 No lab Apr 20 or Apr 21
3 3 Thur Apr 22 ANS] NC] Testing cell viability * M3D3 Lecture
* M3D3 Pre-Lab
3 4 Tue Apr 27 ANS] NC] Preparing cells for analysis * M3D4 Lecture
* M3D4 Pre-Lab
3 5 Thur Apr 29 ANS] NC] Transcript-level analysis * M3D5 Lecture
* M3D5 Pre-Lab
3 6 Tue May 4 ANS] NC] Protein-level analysis Module 2 Final Report Due
* M3D6 Lecture
* M3D6 Pre-Lab
3 7 Thur May 6 ANS] NC] Wrap-up analysis Module 3 Report Due
*Atissa's Lecture
* M3D7 Pre-Lab
3 8 Tue May 11 ANS] NC] Student presentations
Thur May 13 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD