Difference between revisions of "20.109(S10):Post-selection IVT and journal club (Day6)"
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Revision as of 20:31, 24 February 2010
Contents
Introduction
You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentations.
Protocols
Part 1: IVT
Refer to the Day 3 protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL.) Note the time that you put your reactions on the 37 °C heat block.
Part 2: Journal club
We will move to 16-336 for the talks.
For next time
Reagent list
Same as Day 3.