20.109(S07): Start-up expression engineering

From Course Wiki
Revision as of 12:10, 17 December 2006 by Nkuldell (Talk)

Jump to: navigation, search


20.109: Laboratory Fundamentals of Biological Engineering

Macintosh HD-Users-nkuldell-Desktop-20.109template.png

Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Introduction

SAGA intro 1. Gene expression in euk intimately related to chromatin dynamics 2. SAGA is 19 subunit complex req'd for chrom remodel and appropriate gene expression 3. Only 6 of SAGAs 19 subunits essential for viability. 4. Your mission, should you choose to accept it....

Protocols

Part 1: Choosing a SAGA subunit

Part 2: Designing deletion oligos

Part 3: PCR

  1. pRS406 template

DONE!

For next time

Reagents list

Retrieved from "http://engineerbiology.org/w/index.php?title=20.109(S07):_Start-up_expression_engineering&oldid=12660"