20.109(S07): Start-up expression engineering
From Course Wiki
Contents
Introduction
SAGA intro
Protocols
Part 1: Choosing a SAGA subunit
Part 2: Designing deletion oligos
Part 3: PCR
- pRS406 template
DONE!
For next time
Reagents list
Learn a little about the Polymerase Chain Reaction (PCR) since you will perform these reactions next time. It is described in most biology textbooks so you could start there. Alternatively, the internet has some nice animations of the process, for example
- http://www.dnai.org/b/index.html (follow the links through “techniques” and "amplifying DNA”)
- or try: http://www.dnalc.org/shockwave/pcranwhole.html
