Difference between revisions of "20.109(S07): Start-up expression engineering"
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==Introduction== | ==Introduction== | ||
SAGA intro | SAGA intro | ||
+ | 1. Gene expression in euk intimately related to chromatin dynamics | ||
+ | 2. SAGA is 19 subunit complex req'd for chrom remodel and appropriate gene expression | ||
+ | 3. Only 6 of SAGAs 19 subunits essential for viability. | ||
+ | 4. Your mission, should you choose to accept it.... | ||
+ | |||
==Protocols== | ==Protocols== | ||
===Part 1: Choosing a SAGA subunit=== | ===Part 1: Choosing a SAGA subunit=== |
Revision as of 12:10, 17 December 2006
Contents
Introduction
SAGA intro 1. Gene expression in euk intimately related to chromatin dynamics 2. SAGA is 19 subunit complex req'd for chrom remodel and appropriate gene expression 3. Only 6 of SAGAs 19 subunits essential for viability. 4. Your mission, should you choose to accept it....
Protocols
Part 1: Choosing a SAGA subunit
Part 2: Designing deletion oligos
Part 3: PCR
- pRS406 template
DONE!