Difference between revisions of "20.109(S07): Start-up expression engineering"

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==For next time==
 
==For next time==
 
==Reagents list==
 
==Reagents list==
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Learn a little about the Polymerase Chain Reaction (PCR) since you will perform these reactions next time. It is described in most biology textbooks so you could start there. Alternatively, the internet has some nice animations of the process, for example
 
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*http://www.dnai.org/b/index.html (follow the links through “techniques” and "amplifying DNA”)
 
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*or try: http://www.dnalc.org/shockwave/pcranwhole.html
 

Revision as of 03:48, 7 December 2006


20.109: Laboratory Fundamentals of Biological Engineering

Macintosh HD-Users-nkuldell-Desktop-20.109template.png

Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Introduction

SAGA intro

Protocols

Part 1: Choosing a SAGA subunit

Part 2: Designing deletion oligos

Part 3: PCR

  1. pRS406 template

DONE!

For next time

Reagents list

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