Difference between revisions of "20.109(F23):Fall 2023 schedule"

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| R/F Oct 12/13
 
| R/F Oct 12/13
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
| [[20.109(F23):M2D1 | Placeholder]] <br>  
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| [[20.109(F23):M2D1 |Complete in-silico cloning of protein expression plasmid ]]<br>
 
| [[20.109(F23):Homework#Due_M2D1|Homework due]]  
 
| [[20.109(F23):Homework#Due_M2D1|Homework due]]  
 
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| T/W Oct 17/18
 
| T/W Oct 17/18
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
| [[20.109(F23):M2D2 | Placeholder]] <br>  
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| [[20.109(F23):M2D2 |Perform protein purification protocol]] <br>
 
| [[20.109(F23):Homework#Due_M2D2|Homework due]]  
 
| [[20.109(F23):Homework#Due_M2D2|Homework due]]  
 
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| R/F Oct 19/20
 
| R/F Oct 19/20
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
| [[20.109(F23):M2D3 | Placeholder]] <br>  
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| [[20.109(F23):M2D3 |Assess purity and concentration of purified protein]] <br>
 
| [[20.109(F23):Homework#Due_M2D3|Homework due]] <br> [[20.109(F23):Data Summary| <font color =  #227d49 >'''Data Summary revision due'''</font color>]] Sat, Oct 21 at 10 pm <br>  
 
| [[20.109(F23):Homework#Due_M2D3|Homework due]] <br> [[20.109(F23):Data Summary| <font color =  #227d49 >'''Data Summary revision due'''</font color>]] Sat, Oct 21 at 10 pm <br>  
 
|--
 
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| T/W Oct 24/25
 
| T/W Oct 24/25
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| [[20.109(F23):Journal article presentation| <font color =  #227d49>'''Journal article presentations'''</font color>]]
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| R/F Oct 26/27
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| [[20.109(F23):Journal article presentation| <font color =  #227d49 >'''Journal article presentations'''</font color>]]
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| [ Blog post due] Sat, Oct 28 at 10 pm
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| M2D4
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| T/W Oct 30/Nov 1
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [[20.109(F23): <br>  
 
| [[20.109(F23): <br>  
 
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D4|Homework due]]  
 
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D4|Homework due]]  
 
|--
 
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| M2D5
| R/F Oct 26/27
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| R/F Oct 26/27 Nov 2/3
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [[20.109(F23): <br>
 
| [[20.109(F23): <br>
 
| [[20.109(F23):Homework#Due_M2D5|Homework due]]  
 
| [[20.109(F23):Homework#Due_M2D5|Homework due]]  
 
|--
 
|--
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| M2D6
| T/W Oct 30/ Nov 1
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| T/W Nov 7/8
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| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>
| [[20.109(F23):Journal article presentation| <font color =  #227d49>'''Journal article presentations'''</font color>]]
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| [[20.109(F23): <br>
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| [[20.109(F23):Homework#Due_M2D5|Homework due]]
 
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| R/F Nov 2/3
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| T/W Oct 24/25
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| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>
| [[20.109(F23):Journal article presentation| <font color =   #227d49 >'''Journal article presentations'''</font color>]]
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| [[20.109(F23): <br>
| [ Blog post due] Sat, XYZ at 10 pm
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| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D4|Homework due]]
 
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| T/W Nov 7/8
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| R/F Oct 26/27
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br>  
 
| [[20.109(F23): <br>
 
| [[20.109(F23): <br>
| [[20.109(F23):Homework#Due_M2D5|Homework due]]  
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| [[20.109(F23):Homework#Due_M2D5|Homework due]] |--
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| <font color = 999999>R/F Nov 9/10 </font color>
 
| <font color = 999999>R/F Nov 9/10 </font color>

Revision as of 13:41, 24 August 2023

20.109(F23): Laboratory Fundamentals of Biological Engineering

Fa23 banner image.png

Fall 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sep 7/8 NLL
Lecture slides
Orientation and laboratory tour
M1D1 T/W Sep 12/13 BPE
Learn best practices for mammalian cell culture
Orientation quiz
Homework due
M1D2 R/F Sep 14/15 BPE
Prepare and treat cells for γH2AX experiment
Homework due
M1D3 T/W Sep 19/20 BPE
Use immunoflourescence staining to assess γH2AX experiment
Homework due
R/F Sep 21/22 BE Comm Lab Career Day holiday
M1D4 T/W Sep 26/27 BPE
Complete data analysis for γH2AX experiment
Laboratory quiz
Homework due
M1D5 R/F Sep 28/29 BPE
Treat cells for CometChip assay
Homework due
Research talk due Sat, Oct 1 at 10 pm
M1D6 T/W Oct 3/4 BPE
Image and analyze data for CometChip assay
Homework due
M1D7 R/F Oct 5/6 BPE
Examine experimental data using statistical methods
Laboratory quiz
Homework due
T/W Oct 10/11 Indigenous Peoples' Day holiday Data Summary draft due Wed, Oct 11 at 10 pm
[ Blog post due] Thu, Oct 12 at 10 pm
M2D1 R/F Oct 12/13 JCN
Complete in-silico cloning of protein expression plasmid
Homework due
M2D2 T/W Oct 17/18 JCN
Perform protein purification protocol
Homework due
M2D3 R/F Oct 19/20 JCN
Assess purity and concentration of purified protein
Homework due
Data Summary revision due Sat, Oct 21 at 10 pm
T/W Oct 24/25 Journal article presentations
R/F Oct 26/27 Journal article presentations [ Blog post due] Sat, Oct 28 at 10 pm
M2D4 T/W Oct 30/Nov 1 JCN
[[20.109(F23):
Laboratory quiz
Homework due
M2D5 R/F Oct 26/27 Nov 2/3 JCN
[[20.109(F23):
Homework due
M2D6 T/W Nov 7/8 JCN
[[20.109(F23):
Homework due
T/W Oct 24/25 JCN
[[20.109(F23):
Laboratory quiz
Homework due
R/F Oct 26/27 JCN
[[20.109(F23):
Homework due |-- R/F Nov 9/10 Veterans' Day holiday
M2D7 T/W Nov 14/15 JCN
Placeholder
Laboratory quiz
Homework due
M3D1 R/F Nov 16/17 NLL
Brainstorm ideas for Research proposal presentation
Homework due
T/W Nov 21/22 Thanksgiving Day holiday Research article due Mon, Nov 20 at 10 pm
[ Blog post due] Tue, Nov 21 at 10 pm
R/F Nov 23/24 Thanksgiving Day holiday
M3D2 T/W Nov 28/29 AB
Pitch research proposal presentation ideas
M3D3 R/F Nov 30/Dec1 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
M3D4 T/W Dec 5/6 NLL
Participate in Research proposal peer reviews
Homework due
R/F Dec 7/8 Research proposal presentations [ Blog post due] Sat, Dec 9 at 10 pm
T Dec 12 Celebration lunch!