Difference between revisions of "20.109(F17): Notes for M1"
From Course Wiki
(→M1D2) |
(→M1D1) |
||
| Line 4: | Line 4: | ||
===M1D1=== | ===M1D1=== | ||
Per group, aliquot in Tissue Culture, sterile | Per group, aliquot in Tissue Culture, sterile | ||
| − | *14ml of | + | *14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep) |
*3ml of PBS | *3ml of PBS | ||
*1ml of trypsin | *1ml of trypsin | ||
Revision as of 17:49, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep)
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
- Secure line II pen
M1D2
Per group, in main lab
- 1x PBS in glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- 40ml alkaline lysis solution
- 40ml Neutralization buffer
- print a large scheme of the plate so they can keep track of how to load chips
Per group, in Tissue Culture, sterile
- 10 mL MEF media
- 1ml trypsin
- 5ml of PBS
- printout of today's protocol
- print large scheme of the plate to hang in TC so they can keep track of how to load chips
