20.109(F17): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11-12 (???)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)
| MODULE
|
DAY
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
|
R/F Sept 7/8
|
NLL
Orientation lecture
|
Orientation
|
|
|
|
| 1
|
1
|
T/W Sept 12/13
|
BE
|
Prepare microwell array and practice tissue culture
|
Laboratory orientation quiz
Homework due
|
| 1
|
2
|
R/F Sept 14/15
|
NLL
|
Develop experiment to optimize cell loading
|
Homework due
|
| 1
|
3
|
T/W Sept 19/20
|
BE
|
Test role of biochemical factors in genomic stability
|
Laboratory quiz
Homework due
|
| 1
|
4
|
R/F Sept 21/22
|
BE
|
Query inter-individual variability in exposure susceptibility
|
Homework due
|
| 1
|
5
|
T/W Sept 26/27
|
BE
|
Develop approach for sub-nuclear visualization of DNA damage
|
Laboratory quiz
Homework due
|
|
|
|
R/F Sept 28/29
|
BE
|
Lecture, but no laboratory
Career fair student holiday
|
|
| 1
|
6
|
T/W Oct 3/4
|
BE
|
Query DNA repair capacity in tumor cells
|
Laboratory quiz
Homework due
|
| 1
|
7
|
R/F Oct 5/6
|
BE
|
Analysis of sub-nuclear foci
|
Homework due
|
|
|
|
T/W Oct 10/11
|
|
Columbus day holiday
|
Data Summary draft due XX, Oct XX at 10 pm
|
|
|
| 2
|
1
|
R/F Oct 12/13
|
NLL
|
Complete in silico cloning
|
Homework due
Mini-presentation due Xx, Oct XX at 10 pm
|
| 2
|
2
|
T/W Oct 17/18
|
NLL
|
Design gRNA for CRISPRi
|
Homework due
|
| 2
|
3
|
R/F Oct 19/20
|
NLL
|
Generate gRNA plasmid
|
Homework due
Data Summary revision due Xx, Oct XX at 10 pm
|
| 2
|
4
|
T/W Oct 24/25
|
NLL
|
Journal club I
|
Laboratory quiz
Blog post due Xx, Oct XX at 10 pm
|
| 2
|
5
|
R/F Oct 26/27
|
NLL
|
Confirm gRNA sequence
|
Homework due
|
| 2
|
6
|
T/W Oct/Nov 31/1
|
NLL
|
Journal club II
|
Laboratory quiz
|
| 2
|
7
|
R/F Nov 2/3
|
NLL
|
Induce CRISPRi system
|
Homework due
|
| 2
|
8
|
T/W Nov 7/8
|
NLL
|
Measure fermentation products
|
Laboratory quiz
Homework due
|
|
|
|
R/F Nov 9/10
|
|
Veterans' day holiday
|
|
|
|
| 3
|
1
|
T/W Nov 14/15
|
AB
|
Growth of phage materials
|
Homework due
|
| 3
|
2
|
R/F Nov 16/17
|
AB
|
Purify active materials
|
Homework due
Research article due Xx, Nov XX at 10 pm
Blog post due Xx, Nov XX at 10 pm
|
|
|
|
T/W Nov 21/22
|
|
Lecture, but no laboratory
|
|
|
|
|
R/F Nov 23/24
|
|
Thanksgiving holiday
|
|
| 3
|
3
|
T/W Nov 28/29
|
AB
|
Cathode construction
|
Laboratory quiz
Homework due
|
| 3
|
4
|
R/F Nov/Dec 30/1/2
|
AB
|
TEM
|
Homework due
|
| 3
|
5
|
T/W Dec 5/6
|
AB
|
Battery assembly and testing
|
Laboratory quiz
Homework due
Blog post due Xx, Dec X at 10 pm
|
| 3
|
6
|
R/F Dec 7/8
|
|
Research proposal presentations
|
Research proposal presentation slides due Thu/Fri, Dec X/X at 1 pm
|
|
|
|
|
|
T Dec 12
|
|
Feedback and celebratory lunch
|
Biomaterials engineering mini-report due XX, Dec XX at 10 pm
Blog post due Xx, Dec XX at 10pm
|
