Difference between revisions of "20.109(F17): Fall 2017 Schedule"
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| R/F Oct 5/6 | | R/F Oct 5/6 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
| − | | [[20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)| Visualize and analyze data for sub-nuclear foci assay]]<br>[[Media:M1D7_Fa17_LMM_1.pdf| Prelab T/R]][[Media:Fa17_M1D7_JB_marked.pdf| Prelab W/F]] | + | | [[20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)| Visualize and analyze data for sub-nuclear foci assay]]<br>[[Media:M1D7_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M1D7_JB_marked.pdf| Prelab W/F]] |
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M1D7|Homework due]] | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M1D7|Homework due]] | ||
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| 1 | | 1 | ||
| R/F Oct 12/13 | | R/F Oct 12/13 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D1 lecture slides.pdf| Lecture M2D1]] |
| − | | [[20.109(F17):Complete in silico cloning (Day1)| Complete in silico cloning]]<br> | + | | [[20.109(F17):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:M2D1_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D1_Fa17_JB_withLigationCalculation.pdf| Prelab W/F]]<br> |
| [[20.109(F17):Homework#Due_M2D1| Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 14 at 10 pm | | [[20.109(F17):Homework#Due_M2D1| Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 14 at 10 pm | ||
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| 2 | | 2 | ||
| T/W Oct 17/18 | | T/W Oct 17/18 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D2 lecture slides.pdf| Lecture M2D2]] |
| − | | [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br> | + | | [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:M2D2_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D2_Fa17_JB_marked.pdf| Prelab W/F]]<br> |
| [[20.109(F17):Homework#Due_M2D2|Homework due]] | | [[20.109(F17):Homework#Due_M2D2|Homework due]] | ||
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| R/F Oct 19/20 | | R/F Oct 19/20 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| − | | [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> | + | | [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:M2D3_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D3_Fa17_JB_marked.pdf| Prelab W/F]] |
| [[20.109(F17):Homework#Due_M2D3|Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Sun, Oct 22 at 10 pm | | [[20.109(F17):Homework#Due_M2D3|Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Sun, Oct 22 at 10 pm | ||
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| 4 | | 4 | ||
| T/W Oct 24/25 | | T/W Oct 24/25 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D4 lecture slides.pdf| Lecture M2D4]] |
| [[20.109(F17):Journal club I (Day4)| Journal club I]] | | [[20.109(F17):Journal club I (Day4)| Journal club I]] | ||
| [http://be20109f17.blogspot.com/ Blog post due] Mon, Oct 23 at 10 pm | | [http://be20109f17.blogspot.com/ Blog post due] Mon, Oct 23 at 10 pm | ||
| Line 122: | Line 122: | ||
| 5 | | 5 | ||
| R/F Oct 26/27 | | R/F Oct 26/27 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D5 lecture slides.pdf| Lecture M2D5]] |
| − | | [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br> | + | | [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:Fa17_M2D5_EricLehnhardt_marked_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M2D5_JB_marked.pdf| Prelab W/F]] |
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D5|Homework due]] | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D5|Homework due]] | ||
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| 6 | | 6 | ||
| T/W Oct/Nov 31/1 | | T/W Oct/Nov 31/1 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D6 lecture slides.pdf| Lecture M2D6]] |
| [[20.109(F17):Journal club II (Day 6) | Journal club II]] | | [[20.109(F17):Journal club II (Day 6) | Journal club II]] | ||
| | | | ||
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| 7 | | 7 | ||
| R/F Nov 2/3 | | R/F Nov 2/3 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D7 lecture slides.pdf| Lecture M2D7]] |
| − | | [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | + | | [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:M2D7_Prelab_TR_ECL_marked.pdf| Prelab T/R]]<br>[[Media:M2D7_Prelab_WF_JB_marked.pdf| Prelab W/F]]<br> |
| [[20.109(F17):Homework#Due_M2D7|Homework due]] | | [[20.109(F17):Homework#Due_M2D7|Homework due]] | ||
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| 8 | | 8 | ||
| T/W Nov 7/8 | | T/W Nov 7/8 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D8 lecture slides.pdf| Lecture M2D8]] |
| − | | [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br> | + | | [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:M2D8_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D8_Fa17_WF_JB.pdf| Prelab W/F]] |
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D8|Homework due]] | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D8|Homework due]] | ||
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| T/W Nov 14/15 | | T/W Nov 14/15 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| − | | [[20.109(F17):Growth of phage materials (Day1)| Prepare phage materials]]<br> | + | | [[20.109(F17):Growth of phage materials (Day1)| Prepare phage materials]]<br>[[Media:F17_M3D1_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D1_WF_JB_marked.pdf| Prelab W/F]]<br> |
| [[20.109(F17):Homework#Due_M3D1|Homework due]] <br> | | [[20.109(F17):Homework#Due_M3D1|Homework due]] <br> | ||
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| R/F Nov 16/17 | | R/F Nov 16/17 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| − | | [[20.109(F17):Purify active materials (Day2)| Purify active material for cathode construction]]<br> | + | | [[20.109(F17):Purify active materials (Day2)| Purify active material for cathode construction]]<br>[[Media:F17_M3D2_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D2_JB_marked.pdf| Prelab W/F]]<br> |
| [[20.109(F17):Homework#Due_M3D2|Homework due]]<br> | | [[20.109(F17):Homework#Due_M3D2|Homework due]]<br> | ||
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| | | | ||
| T/W Nov 21/22 | | T/W Nov 21/22 | ||
| − | | | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:BATTERIES 20.109 Fall 2017.pdf| Lecture]] |
| <font color = #31B404>'''Lecture, but no laboratory'''</font color> | | <font color = #31B404>'''Lecture, but no laboratory'''</font color> | ||
| [[20.109(F17): Research article| Research article due]] Mon, Nov 20 at 10 pm <br>[http://be20109f17.blogspot.com/ Blog post due] Tue, Nov 21 at 10 pm | | [[20.109(F17): Research article| Research article due]] Mon, Nov 20 at 10 pm <br>[http://be20109f17.blogspot.com/ Blog post due] Tue, Nov 21 at 10 pm | ||
| Line 187: | Line 187: | ||
| 3 | | 3 | ||
| T/W Nov 28/29 | | T/W Nov 28/29 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB] |
| − | | [[20.109(F17):Cathode construction (Day3)| Construct cathode with active material]]<br> | + | | [[20.109(F17):Cathode construction (Day3)| Construct cathode with active material]]<br>[[Media:F17_M3D3_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D3_JB_marked.pdf| Prelab W/F]]<br> |
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D3| Homework due]] | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D3| Homework due]] | ||
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| 4 | | 4 | ||
| R/F Nov/Dec 30/1 | | R/F Nov/Dec 30/1 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB] <br>[[Media:20.109 TEM Fall 2017.pdf| Lecture]] |
| − | | [[20.109(F17):Visualize active material using TEM (Day4)| Visualize active material using TEM]]<br> | + | | [[20.109(F17):Visualize active material using TEM (Day4)| Visualize active material using TEM]]<br>[[Media:Fa17_M3D4_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M3D4_JB_marked.pdf| Prelab W/F]] |
| [[20.109(F17):Homework#Due_M3D4|Homework due]] | | [[20.109(F17):Homework#Due_M3D4|Homework due]] | ||
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| 5 | | 5 | ||
| T/W Dec 5/6 | | T/W Dec 5/6 | ||
| − | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:Battery data 20.109 Fall 2017.pdf| Lecture]] |
| − | | [[20.109(F17):Assemble and test batteries (Day5)| Assemble and test batteries]]<br> | + | | [[20.109(F17):Assemble and test batteries (Day5)| Assemble and test batteries]]<br>[[Media:Fa17_M3D5_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M3D5_JB.pdf| Prelab W/F]] |
| − | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D5|Homework due]] | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D5|Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
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| | | | ||
| [[20.109(F17):Research proposal presentation| Research proposal presentations]] | | [[20.109(F17):Research proposal presentation| Research proposal presentations]] | ||
| − | | [[20.109(F17):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 7/8 at 1 pm | + | | [[20.109(F17):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 7/8 at 1 pm<br>[http://be20109f17.blogspot.com/ Blog post due] Fri, Dec 8 at 10 pm |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
Latest revision as of 20:44, 6 December 2017
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11-12 (16-220)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)
