Difference between revisions of "20.109(F17): Fall 2017 Schedule"

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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>
 
| [[20.109(F17):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>
| [[Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Xx, Oct XX at 10 pm
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| [[20.109(F17):Homework#Due_M2D1| Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Xx, Oct XX at 10 pm
 
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>
 
| [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>
| [[Homework due]]
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| [[20.109(F17):Homework#Due_M2D2|Homework due]]
 
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>
 
| [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>
| [[Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Xx, Oct XX at 10 pm
+
| [[20.109(F17):Homework#Due_M2D3|Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Xx, Oct XX at 10 pm
 
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>
 
| [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>
| [[Homework due]]
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| [[20.109(F17):Homework#Due_M2D5|Homework due]]
 
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>
 
| [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>
| [[Homework due]]
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| [[20.109(F17):Homework#Due_M2D7|Homework due]]
 
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>
 
| [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[Homework due]]
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| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D8|Homework due]]
 
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| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [[20.109(F17):Growth of phage materials (Day1)| Prepare phage materials]]<br>
 
| [[20.109(F17):Growth of phage materials (Day1)| Prepare phage materials]]<br>
| [[Homework due]] <br>  
+
| [[20.109(F17):Homework#Due_M3D1|Homework due]] <br>  
 
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| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [[20.109(F17):Purify active materials (Day2)| Purify active material for cathode construction]]<br>
 
| [[20.109(F17):Purify active materials (Day2)| Purify active material for cathode construction]]<br>
| [[Homework due]]<br>[[20.109(F16): Research article| Research article due]] Xx, Nov XX at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Nov XX at 10 pm
+
| [[20.109(F17):Homework#Due_M3D2|Homework due]]<br>[[20.109(F16): Research article| Research article due]] Xx, Nov XX at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Nov XX at 10 pm
 
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| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [[20.109(F17):Cathode construction (Day3)| Construct cathode with active material]]<br>
 
| [[20.109(F17):Cathode construction (Day3)| Construct cathode with active material]]<br>
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]]
+
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D3| Homework due]]
 
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| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [[20.109(F17):TEM (Day4)| Visualize active material using TEM]]<br>
 
| [[20.109(F17):TEM (Day4)| Visualize active material using TEM]]<br>
| [[Homework due]]
+
| [[20.109(F17):Homework#Due_M3D4|Homework due]]
 
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| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [[20.109(F17):Battery assembly and testing (Day5)| Assemble and test batteries]]<br>
 
| [[20.109(F17):Battery assembly and testing (Day5)| Assemble and test batteries]]<br>
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Dec X at 10 pm
+
| <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D5|Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Dec X at 10 pm
 
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Revision as of 17:12, 22 August 2017

20.109(F17): Laboratory Fundamentals of Biological Engineering

Fa17 banner image.png

Fa17 Schedule        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11-12 (???)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 7/8 NLL
Orientation lecture
Orientation
1 1 T/W Sept 12/13 BE Prepare microwell array and practice tissue culture
Laboratory orientation quiz
Homework due
1 2 R/F Sept 14/15 NLL Develop experiment to optimize cell loading
Homework due
1 3 T/W Sept 19/20 BE Test role of biochemical factors in genomic stability
Laboratory quiz
Homework due
1 4 R/F Sept 21/22 BE Query inter-individual variability in exposure susceptibility
Homework due
1 5 T/W Sept 26/27 BE Develop approach for sub-nuclear visualization of DNA damage Laboratory quiz
Homework due
R/F Sept 28/29 BE Lecture, but no laboratory
Career fair student holiday
1 6 T/W Oct 3/4 BE Query DNA repair capacity in tumor cells
Laboratory quiz
Homework due
1 7 R/F Oct 5/6 BE Analysis of sub-nuclear foci
Homework due
T/W Oct 10/11 Columbus day holiday Data Summary draft due XX, Oct XX at 10 pm
2 1 R/F Oct 12/13 NLL
Complete in silico cloning
Homework due
Mini-presentation due Xx, Oct XX at 10 pm
2 2 T/W Oct 17/18 NLL
Design gRNA for CRISPRi
Homework due
2 3 R/F Oct 19/20 NLL
Generate gRNA plasmid
Homework due
Data Summary revision due Xx, Oct XX at 10 pm
2 4 T/W Oct 24/25 NLL
Journal club I Laboratory quiz
Blog post due Xx, Oct XX at 10 pm
2 5 R/F Oct 26/27 NLL
Confirm gRNA sequence
Homework due
2 6 T/W Oct/Nov 31/1 NLL
Journal club II Laboratory quiz
2 7 R/F Nov 2/3 NLL
Induce CRISPRi system
Homework due
2 8 T/W Nov 7/8 NLL
Measure fermentation products
Laboratory quiz
Homework due
R/F Nov 9/10 Veterans' day holiday
3 1 T/W Nov 14/15 AB Prepare phage materials
Homework due
3 2 R/F Nov 16/17 AB Purify active material for cathode construction
Homework due
Research article due Xx, Nov XX at 10 pm
Blog post due Xx, Nov XX at 10 pm
T/W Nov 21/22 Lecture, but no laboratory
R/F Nov 23/24 Thanksgiving holiday
3 3 T/W Nov 28/29 AB Construct cathode with active material
Laboratory quiz
Homework due
3 4 R/F Nov/Dec 30/1/2 AB Visualize active material using TEM
Homework due
3 5 T/W Dec 5/6 AB Assemble and test batteries
Laboratory quiz
Homework due
Blog post due Xx, Dec X at 10 pm
3 6 R/F Dec 7/8 Research proposal presentations Research proposal presentation slides due Thu/Fri, Dec X/X at 1 pm
T Dec 12 Feedback and celebratory lunch Biomaterials engineering mini-report due XX, Dec XX at 10 pm
Blog post due Xx, Dec XX at 10pm