Difference between revisions of "20.109(F17): Fall 2017 Schedule"

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| 1
 
| 1
 
| R/F Oct 12/13
 
| R/F Oct 12/13
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D1 lecture slides.pdf|M2D1 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:Fa16_M2D1_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D1_161014_marked.pdf| Prelab W/F]]
+
| [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>
| [[20.109(F16):Homework#Due_M2D1| Homework due]]<br>[[20.109(F16): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 15 at 10 pm
+
| [[Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Xx, Oct XX at 10 pm
 
|--
 
|--
 
| 2
 
| 2
 
| 2
 
| 2
| T/W Oct 18/19
+
| T/W Oct 17/18
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media:Fa16 M2D2 lecture slides.pdf|M2D2 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:M2D2_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D2_161019_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>
| [[20.109(F16):Homework#Due_M2D2| Homework due]]
+
| [[Homework due]]
 
|--
 
|--
 
| 2
 
| 2
 
| 3
 
| 3
| R/F Oct 20/21
+
| R/F Oct 19/20
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D3 lecture slides.pdf|M2D3 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:M2D3_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D3_161021_marked.pdf| Prelab W/F]]<br>[[Media:Fa16 M2D3 journal article discussion.pdf|Journal article discussion]]
+
| [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>
| [[20.109(F16):Homework#Due_M2D3| Homework due]]<br>[[20.109(F16): M1 Data Summary| Data Summary revision due]] Mon, Oct 24 at 5 pm
+
| [[Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Xx, Oct XX at 10 pm
 
|--
 
|--
 
| 2
 
| 2
 
| 4
 
| 4
| T/W Oct 25/26
+
| T/W Oct 24/25
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D4 lecture slides student.pdf|M2D4 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Journal club I (Day4)| Journal club I]]
+
| [[20.109(F17):Journal club I (Day4)| Journal club I]]
| <font color=#04B45F>'''Lab quiz'''</font color><br>[http://be20109f16.blogspot.com/ Blog post due] Tue, Oct 25 at 5pm
+
| <font color=#0000FF>'''Laboratory quiz'''</font color><br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Oct XX at 10 pm
 
|--
 
|--
 
| 2
 
| 2
 
| 5
 
| 5
| R/F Oct 27/28
+
| R/F Oct 26/27
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D5 lecture slides.pdf|M2D5 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:M2D5 Pre-Lab TR.pdf| Prelab T/R]]<br>[[Media:Fa16 M2D5 EmilyClark 161027 pptx.pdf| Prelab W/F]]
+
| [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>
| [[20.109(F16):Homework#Due_M2D5| Homework due]]
+
| [[Homework due]]
 
|--
 
|--
 
| 2
 
| 2
 
| 6
 
| 6
| T/W Nov 1/2
+
| T/W Oct/Nov 31/1
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D6 lecture slides.pdf|M2D6 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Journal club II (Day 6) | Journal club II]]
+
| [[20.109(F17):Journal club II (Day 6) | Journal club II]]
| <font color=#04B45F>'''Lab quiz'''</font color><br>
+
| <font color=#0000FF>'''Laboratory quiz'''</font color><br>
 
|--
 
|--
 
| 2
 
| 2
 
| 7
 
| 7
| R/F Nov 3/4
+
| R/F Nov 2/3
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D7 lecture slides.pdf|M2D7 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:M2D7_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D7_161104_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>
| [[20.109(F16):Homework#Due_M2D7| Homework due]]
+
| [[Homework due]]
 
|--
 
|--
 
| 2
 
| 2
 
| 8
 
| 8
| T/W Nov 8/9
+
| T/W Nov 7/8
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D8 lecture slides.pdf|M2D8 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F16)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:M2D8_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D8_161109_marked.pdf| Prelab W/F]]
+
| [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M2D8| Homework due]]
+
| <font color=#0000FF>'''Laboratory quiz'''</font color><br>[[Homework due]]
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
| <font color = 999999>R/F Nov 10/11</font color>
+
| <font color = 999999>R/F Nov 9/10</font color>
 
|  
 
|  
 
| <font color = 999999>Veterans' day holiday</font color>
 
| <font color = 999999>Veterans' day holiday</font color>
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| 3
 
| 3
 
| 1
 
| 1
| T/W Nov 15/16
+
| T/W Nov 14/15
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
| [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]]<br>[[Media:Fa16 M3D1 prelab slides.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D1_161116_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Growth of phage materials (Day1)| Growth of phage materials]]<br>
| [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br>  
+
| [[Homework due]] <br>  
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
| R/F Nov 17/18
+
| R/F Nov 16/17
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
| [[20.109(F16):Purify active materials (Day2)| Purify active materials]]<br>[[Media:Fa16_M3D2_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D2_161118_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Purify active materials (Day2)| Purify active materials]]<br>
| [[20.109(F16):Homework#Due_M3D2| Homework due]]<br>[[20.109(F16): Research article| Research article due]] Sun, Nov 20 at 5 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Mon, Nov 21 at 5pm
+
| [[Homework due]]<br>[[20.109(F16): Research article| Research article due]] Xx, Nov XX at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Nov XX at 10 pm
 
|--
 
|--
 
|
 
|
 
|
 
|
| T/W Nov 22/23
+
| T/W Nov 21/22
 
|
 
|
| <font color = #FF0000>Lecture, but no laboratory</font color>
+
| <font color = #31B404>'''Lecture, but no laboratory'''</font color>
 
|  
 
|  
 
|--  
 
|--  
 
|
 
|
 
|
 
|
| <font color = 999999>R/F Nov 24/25</font color>
+
| <font color = 999999>R/F Nov 23/24</font color>
 
|
 
|
 
| <font color = 999999>Thanksgiving holiday</font color>
 
| <font color = 999999>Thanksgiving holiday</font color>
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| 3
 
| 3
 
| 3
 
| 3
| T/W Nov 29/30
+
| T/W Nov 28/29
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
| [[20.109(F16):Cathode construction (Day3)| Cathode construction]]<br>[[Media:Fa16_M3D3_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D3_161130_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Cathode construction (Day3)| Cathode construction]]<br>
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]]
+
| <font color=#0000FF>'''Laboratory quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 4
 
| 4
| R/F Dec 1/2  
+
| R/F Nov/Dec 30/1/2  
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
| [[20.109(F16):TEM (Day4)| TEM]]<br>[[Media:Fa16_M3D4_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D4_161202_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):TEM (Day4)| TEM]]<br>
| [[20.109(F16):Homework#Due_M3D4| Homework due]]
+
| [[Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
| T/W Dec 6/7  
+
| T/W Dec 5/6  
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
| [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]]<br>[[Media:Fa16_M3D5_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D5_161207_marked.pdf| Prelab W/F]]
+
| [[20.109(F17):Battery assembly and testing (Day5)| Battery assembly and testing]]<br>
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 7 at 10pm
+
| <font color=#0000FF>'''Laboratory quiz'''</font color><br>[[Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Dec X at 10 pm
 
|--
 
|--
 
| 3
 
| 3
 
| 6
 
| 6
| R/F Dec 8/9
+
| R/F Dec 7/8
 
|  
 
|  
| [[20.109(F16):Research proposal presentation| Research proposal presentations]]
+
| [[20.109(F17):Research proposal presentation| Research proposal presentations]]
| [[20.109(F16):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 8/9 at 1 pm
+
| [[20.109(F17):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec X/X at 1 pm
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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|  
 
|  
 
|  
 
|  
| T Dec 13
+
| T Dec 12
 
|  
 
|  
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color>
+
| <font color = #31B404>'''Feedback and celebratory lunch'''</font color>
| [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 14 at 10pm
+
| [[20.109(F17): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] XX, Dec XX at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Xx, Dec XX at 10pm
 
|}
 
|}
 
</div>
 
</div>

Revision as of 17:15, 15 June 2017

20.109(F17): Laboratory Fundamentals of Biological Engineering

Fa17 banner image.png

Fa17 Schedule        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11-12 (???)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 7/8 NLL
Orientation lecture
Orientation
1 1 T/W Sept 12/13 BE Prepare microwell array and practice tissue culture
Laboratory orientation quiz
Homework due
1 2 R/F Sept 14/15 BE Develop experiment to test loading variables and quantify growth rate
Homework due
1 3 T/W Sept 19/20 BE Test role of biochemical factors in genomic stability
Laboratory quiz
Homework due
1 4 R/F Sept 21/22 BE Query inter-individual variability in exposure susceptibility
Homework due
1 5 T/W Sept 26/27 NLL Develop approach for sub-nuclear visualization of DNA damage Laboratory quiz
Homework due
R/F Sept 28/29 BE Lecture, but no laboratory
Career fair student holiday
1 6 T/W Oct 3/4 BE Query DNA repair capacity in tumor cells
Laboratory quiz
Homework due
1 7 R/F Oct 5/6 BE Analysis of sub-nuclear foci
Homework due
T/W Oct 10/11 Columbus day holiday Data Summary draft due XX, Oct XX at 10 pm
2 1 R/F Oct 12/13 NLL
Complete in silico cloning
Homework due
Mini-presentation due Xx, Oct XX at 10 pm
2 2 T/W Oct 17/18 NLL
Design gRNA for CRISPRi
Homework due
2 3 R/F Oct 19/20 NLL
Generate gRNA plasmid
Homework due
Data Summary revision due Xx, Oct XX at 10 pm
2 4 T/W Oct 24/25 NLL
Journal club I Laboratory quiz
Blog post due Xx, Oct XX at 10 pm
2 5 R/F Oct 26/27 NLL
Confirm gRNA sequence
Homework due
2 6 T/W Oct/Nov 31/1 NLL
Journal club II Laboratory quiz
2 7 R/F Nov 2/3 NLL
Induce CRISPRi system
Homework due
2 8 T/W Nov 7/8 NLL
Measure fermentation products
Laboratory quiz
Homework due
R/F Nov 9/10 Veterans' day holiday
3 1 T/W Nov 14/15 AB Growth of phage materials
Homework due
3 2 R/F Nov 16/17 AB Purify active materials
Homework due
Research article due Xx, Nov XX at 10 pm
Blog post due Xx, Nov XX at 10 pm
T/W Nov 21/22 Lecture, but no laboratory
R/F Nov 23/24 Thanksgiving holiday
3 3 T/W Nov 28/29 AB Cathode construction
Laboratory quiz
Homework due
3 4 R/F Nov/Dec 30/1/2 AB TEM
Homework due
3 5 T/W Dec 5/6 AB Battery assembly and testing
Laboratory quiz
Homework due
Blog post due Xx, Dec X at 10 pm
3 6 R/F Dec 7/8 Research proposal presentations Research proposal presentation slides due Thu/Fri, Dec X/X at 1 pm
T Dec 12 Feedback and celebratory lunch Biomaterials engineering mini-report due XX, Dec XX at 10 pm
Blog post due Xx, Dec XX at 10pm