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| | | R/F Sept 28/29 | | | R/F Sept 28/29 |
| | | [http://be.mit.edu/directory/bevin-p-engelward BE] | | | [http://be.mit.edu/directory/bevin-p-engelward BE] |
| − | | Lecture, but no laboratory</font color><br><font color = 999999>Career fair student holiday</font color><br> | + | | <font color = #31B404>Lecture, but no laboratory</font color><br><font color = 999999>Career fair student holiday</font color><br> |
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| | | R/F Oct 5/6 | | | R/F Oct 5/6 |
| | | [http://be.mit.edu/directory/bevin-p-engelward BE] | | | [http://be.mit.edu/directory/bevin-p-engelward BE] |
| − | | [[20.109(F16):Data analysis (Day7)| Analysis of sub-nuclear foci]]<br>[[Media:M1D7_Fa16_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D7_161007_marked.pdf| Prelab W/F]] | + | | [[20.109(F17):Data analysis (Day7)| Analysis of sub-nuclear foci]]<br> |
| − | | [[20.109(F16):Homework#Due_M1D7| Homework due]] | + | | [[Homework due]] |
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Revision as of 16:58, 15 June 2017
20.109(F17): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11-12 (???)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)
| MODULE
|
DAY
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
|
R/F Sept 7/8
|
NLL
Orientation lecture
|
Orientation
|
|
|
|
| 1
|
1
|
T/W Sept 12/13
|
BE
|
Prepare microwell array and practice tissue culture
|
Lab orientation quiz
Homework due
|
| 1
|
2
|
R/F Sept 14/15
|
BE
|
Develop experiment to test loading variables and quantify growth rate
|
Homework due
|
| 1
|
3
|
T/W Sept 19/20
|
BE
|
Test role of biochemical factors in genomic stability
|
Lab quiz
Homework due
|
| 1
|
4
|
R/F Sept 21/22
|
BE
|
Query inter-individual variability in exposure susceptibility
|
Homework due
|
| 1
|
5
|
T/W Sept 26/27
|
NLL
|
Develop approach for sub-nuclear visualization of DNA damage
|
Lab quiz
Homework due
|
|
|
|
R/F Sept 28/29
|
BE
|
Lecture, but no laboratory
Career fair student holiday
|
|
| 1
|
6
|
T/W Oct 3/4
|
BE
|
Query DNA repair capacity in tumor cells
|
Lab quiz
Homework due
|
| 1
|
7
|
R/F Oct 5/6
|
BE
|
Analysis of sub-nuclear foci
|
Homework due
|
|
|
|
T/W Oct 10/11
|
|
Columbus day holiday
|
Data Summary draft due XX, Oct XX at 10 pm
|
|
|
| 2
|
1
|
R/F Oct 12/13
|
NLL
M2D1 lecture
|
Complete in silico cloning
Prelab T/R
Prelab W/F
|
Homework due
Mini-presentation due Sat, Oct 15 at 10 pm
|
| 2
|
2
|
T/W Oct 18/19
|
NLL
M2D2 lecture
|
Design gRNA for CRISPRi
Prelab T/R
Prelab W/F
|
Homework due
|
| 2
|
3
|
R/F Oct 20/21
|
NLL
M2D3 lecture
|
Generate gRNA plasmid
Prelab T/R
Prelab W/F
Journal article discussion
|
Homework due
Data Summary revision due Mon, Oct 24 at 5 pm
|
| 2
|
4
|
T/W Oct 25/26
|
NLL
M2D4 lecture
|
Journal club I
|
Lab quiz
Blog post due Tue, Oct 25 at 5pm
|
| 2
|
5
|
R/F Oct 27/28
|
NLL
M2D5 lecture
|
Confirm gRNA sequence
Prelab T/R
Prelab W/F
|
Homework due
|
| 2
|
6
|
T/W Nov 1/2
|
NLL
M2D6 lecture
|
Journal club II
|
Lab quiz
|
| 2
|
7
|
R/F Nov 3/4
|
NLL
M2D7 lecture
|
Induce CRISPRi system
Prelab T/R
Prelab W/F
|
Homework due
|
| 2
|
8
|
T/W Nov 8/9
|
NLL
M2D8 lecture
|
Measure fermentation products
Prelab T/R
Prelab W/F
|
Lab quiz
Homework due
|
|
|
|
R/F Nov 10/11
|
|
Veterans' day holiday
|
|
|
|
| 3
|
1
|
T/W Nov 15/16
|
AB
|
Growth of phage materials
Prelab T/R
Prelab W/F
|
Homework due
|
| 3
|
2
|
R/F Nov 17/18
|
AB
|
Purify active materials
Prelab T/R
Prelab W/F
|
Homework due
Research article due Sun, Nov 20 at 5 pm
Blog post due Mon, Nov 21 at 5pm
|
|
|
|
T/W Nov 22/23
|
|
Lecture, but no laboratory
|
|
|
|
|
R/F Nov 24/25
|
|
Thanksgiving holiday
|
|
| 3
|
3
|
T/W Nov 29/30
|
AB
|
Cathode construction
Prelab T/R
Prelab W/F
|
Lab quiz
Homework due
|
| 3
|
4
|
R/F Dec 1/2
|
AB
|
TEM
Prelab T/R
Prelab W/F
|
Homework due
|
| 3
|
5
|
T/W Dec 6/7
|
AB
|
Battery assembly and testing
Prelab T/R
Prelab W/F
|
Lab quiz
Homework due
Blog post due Wed, Dec 7 at 10pm
|
| 3
|
6
|
R/F Dec 8/9
|
|
Research proposal presentations
|
Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm
|
|
|
|
|
|
T Dec 13
|
|
Feedback and celebratory lunch
|
Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm
Blog post due Wed, Dec 14 at 10pm
|
