20.109(F16): TA notes for M12
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M2D1
Per group, aliquot
- 30 μL nuclease-free water
- 5 μL pdCas9 (at 0.5)
On front bench, have
- restriction enzymes in -20 °C block
- NEB buffers
- list of 20.109-owned restriction enzymes
Fa16: all done by 5pm
M2D2
Per group,
- pour 1/2 agarose gel (only 5 lanes needed)
- thaw 4 samples of confirmation digests
- aliquot 10 μL of 6X loading buffer
- thaw 20 μL of 1 kb ladder