20.109(F16): Fall 2016 schedule
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
R/F Sept 8/9 | NLL |
Orientation |
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1 | 1 | T/W Sept 13/14 | BE | Prepare comet chips |
Lab orientation quiz Homework due | |
1 | 2 | R/F Sept 15/16 | BE | Test comet chip loading variables |
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1 | 3 | T/W Sept 20/21 | BE | Measure DNA damage with comet chip | Lab quiz Homework due | |
R/F Sept 22/23 | Career fair student holiday | |||||
1 | 4 | T/W Sept 27/28 | NLL | Asses DNA repair variability with comet chip | Lab quiz Homework due | |
1 | 5 | R/F Sept 29/30 | BE | Seed cells for immuno-fluorescence assay | Homework due | |
1 | 6 | T/W Oct 4/5 | BE | Complete immuno-fluorescence assay | Lab quiz Homework due | |
1 | 7 | R/F Oct 6/7 | BE | Data analysis | Homework due | |
T/W Oct 11/12 | Columbus day holiday | Data Summary draft due Wed, Oct 12 at 5 pm | ||||
2 | 1 | R/F Oct 13/14 | NLL | Complete in silico cloning | Homework due Mini-presentation due Sat, Oct 15 at 10 pm | |
2 | 2 | T/W Oct 18/19 | NLL | Design gRNA for CRISPRi | Lab quiz Homework due | |
2 | 3 | R/F Oct 20/21 | NLL | Generate gRNA plasmid | Homework due Data Summary revision due Mon, Oct 24 at 5 pm | |
2 | 4 | T/W Oct 25/26 | NLL | Journal club I | Lab quiz | |
2 | 5 | R/F Oct 27/28 | NLL | Confirm gRNA sequence | Homework due | |
2 | 6 | T/W Nov 1/2 | NLL | Journal club II | Lab quiz | |
2 | 7 | R/F Nov 3/4 | NLL | Induce CRISPRi system | Homework due | |
2 | 8 | T/W Nov 8/9 | NLL | Measure fermentation products] | Lab quiz Homework due | |
R/F Nov 10/11 | Veterans' day holiday | |||||
3 | 1 | T/W Nov 15/16 | AB | Growth of phage materials | Homework due Protein engineering report due Sun, Nov 20 at 5 pm | |
3 | 2 | R/F Nov 17/18 | AB | Purify active materials | Homework due | |
T/W Nov 22/23 | AB | Lecture, but no laboratory | ||||
R/F Nov 24/25 | Thanksgiving holiday | |||||
3 | 3 | T/W Nov 29/30 | AB | Cathode construction | Lab quiz Homework due | |
3 | 4 | R/F Dec 1/2 | AB | TEM | Homework due | |
3 | 5 | T/W Dec 6/7 | AB | Battery assembly and testing | Lab quiz Homework due | |
3 | 6 | R/F Dec 8/9 | Research proposal presentations | Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm | ||
T Dec 13 | Feedback and celebratory lunch | Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm |