20.109(F16): Fall 2016 schedule
Welcome to 20.109! For some of you this will be the first time in a research lab and for others it will not; either way, it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping, and publicly presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | DATA / NOTES |
| R/F Sept 10/11 | NLL |
Orientation |
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| 1 | 1 | T/W Sept 15/16 | BE | DNA engineering using PCR |
Lab orientation quiz Homework due |
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| 1 | 2 | R/F Sept 17/18 | BE | Clean and cut DNA |
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| 1 | 3 | T/W Sept 22/23 | BE | Agarose gel electrophoresis | Homework due | |
| 1 | 4 | R/F Sept 24/25 | BE | Ligation & transformation | Lab quiz Homework due |
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| 1 | 5 | T/W Sept 29/30 | NLL | Examine candidate clones and tissue culture | Homework due | |
| 1 | 6 | R/F Oct 1/2 | BE | Lipofection | Homework due | |
| 1 | 7 | T/W Oct 6/7 | BE | Data analysis | Lab quiz Homework due |
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| 2 | 1 | NLL | ||||
| 3 | 1 | R/F Nov 12/13 | AB | Growth of phage materials | Homework due Protein engineering report due Sun, Nov 15 at 5 pm |
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| 3 | 2 | T/W Nov 17/18 | AB | Purify active materials | Homework due | |
| 3 | 3 | R/F Nov 19/20 | AB | Cathode construction | Lab quiz Homework due |
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| T/W Nov 24/25 | AB | Lecture, but no lab | ||||
| R/F Nov 26/27 | Thanksgiving holiday | |||||
| 3 | 4 | T/W Dec 1/2 | AB | TEM | Homework due | |
| 3 | 5 | R/F Dec 3/4 | AB | Battery assembly and testing | Lab quiz Homework due Biomaterials engineering mini-report due Thu/Fri, Dec 3/4 at 10 pm |
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| 3 | 6 | T/W Dec 8/9 | Research proposal presentations | Research proposal presentation slides due Tue/Wed, Dec 8/9 at 1 pm | ||
| R Dec 10 | Feedback and celebratory lunch | |||||
