Difference between revisions of "20.109(F16): Fall 2016 schedule"

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{{Template:20.109(F16)}}
 
{{Template:20.109(F16)}}
 
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Welcome to 20.109! For some of you this will be the first time in a research lab and for others it will not; either way, it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping, and publicly presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
+
 
 +
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
 +
 
 +
'''Schedule details:'''<br>
 +
'''Lecture:'''  T/R 11-12 (16-220)  <br>
 +
'''Laboratory sections:'''      T/R 1-5 or W/F 1-5 (56-322)<br>
  
 
{| border=1px
 
{| border=1px
Line 10: Line 15:
 
|'''LABORATORY EXPERIMENTS'''
 
|'''LABORATORY EXPERIMENTS'''
 
|'''ASSIGNMENTS'''
 
|'''ASSIGNMENTS'''
−
|'''DATA / NOTES'''
 
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
−
| R/F Sept 10/11  
+
| R/F Sept 8/9  
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 Orientation lecture.pdf| Orientation lecture]]
−
| [[20.109(F16):Lab tour | Orientation]]<br>
+
| [[20.109(F16):Lab tour | Orientation]]<br>[[Media:MOD0_F16_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M0D0_160909_marked.pdf| Prelab W/F]]
−
|
+
 
|
 
|
 
|--
 
|--
Line 24: Line 27:
 
| 1
 
| 1
 
| 1
 
| 1
−
| T/W Sept 15/16
+
| T/W Sept 13/14
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):DNA engineering using PCR (Day1) | DNA engineering using PCR]]<br>
+
| [[20.109(F16):Prepare comet chips (Day1) | Prepare microwell array and practice tissue culture]]<br>[[Media:M1D1_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D1_160914_marked.pdf| Prelab W/F]]
 
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]]
 
| <font color=#04B45F>'''Lab orientation quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D1| Homework due]]
−
|
 
 
|--
 
|--
 
| 1
 
| 1
 
| 2
 
| 2
−
| R/F Sept 17/18    
+
| R/F Sept 15/16    
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):Clean and cut DNA (Day2) | Clean and cut DNA]]<br>  
+
| [[20.109(F16):Test comet chip loading variables (Day2) | Develop experiment to test loading variables and quantify growth rate]]<br>[[Media:M1D2_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D2_160916_marked.pdf| Prelab W/F]]
−
|
+
|[[20.109(F16):Homework#Due_M1D2| Homework due]]
−
|
+
 
|--
 
|--
 
| 1
 
| 1
 
| 3
 
| 3
−
| T/W Sept 22/23  
+
| T/W Sept 20/21  
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):Agarose gel electrophoresis (Day3)| Agarose gel electrophoresis]]
+
| [[20.109(F16):Measure DNA damage with comet chip (Day3)| Test role of biochemical factors in genomic stability]]<br>[[Media:M1D3_Fa16_LMM_2.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D3_160921_marked.pdf| Prelab W/F]]
−
| [[20.109(F16):Homework#Due_M1D3| Homework due]]
+
| <font color=#04B45F>'''Lab quiz'''</font color><br> [[20.109(F16):Homework#Due_M1D3| Homework due]]
 +
|--
 +
|
 +
|
 +
| R/F Sept 22/23
 +
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 +
| <font color = #FF0000>Lecture, but no laboratory</font color><br><font color = 999999>Career fair student holiday</font color>
 
|
 
|
 
|--
 
|--
 
| 1
 
| 1
 
| 4
 
| 4
−
| R/F Sept 24/25
+
| T/W Sept 27/28
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]
−
| [[20.109(F16):DNA ligation & transformation (Day4)| Ligation & transformation]]
+
| [[20.109(F16):Asses DNA repair variability with comet chip (Day4)| Query inter-individual variability in exposure susceptibility]]<br>[[Media:M1D4_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D4_160928_marked.pdf| Prelab W/F]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]]
 
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D4| Homework due]]
−
|
 
 
|--
 
|--
 
| 1
 
| 1
 
| 5
 
| 5
−
| T/W Sept 29/30  
+
| R/F Sept 29/30  
−
| [http://be.mit.edu/directory/noreen-lyell NLL]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):Examine candidate clones & tissue culture (Day5)| Examine candidate clones and tissue culture]]
+
| [[20.109(F16):Seed cells for immuno-flourescence assay (Day5)| Develop approach for sub-nuclear visualization of DNA damage]]<br>[[Media:M1D5_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D5_160930_marked.pdf| Prelab W/F]]
 
| [[20.109(F16):Homework#Due_M1D5| Homework due]]
 
| [[20.109(F16):Homework#Due_M1D5| Homework due]]
−
|
 
 
|--
 
|--
 
| 1
 
| 1
 
| 6
 
| 6
−
| R/F Oct 1/2
+
| T/W Oct 4/5
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):Lipofection (Day6)| Lipofection]]
+
| [[20.109(F16):Complete immuno-fluorescence assay (Day6)| Query DNA repair capacity in tumor cells]]<br>[[Media:M1D6_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D6_161005_marked.pdf| Prelab W/F]]
−
| [[20.109(F16):Homework#Due_M1D6| Homework due]]
+
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D6| Homework due]]
−
|
+
 
|--
 
|--
 
| 1
 
| 1
 
| 7
 
| 7
−
| T/W Oct 6/7
+
| R/F Oct 6/7
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
−
| [[20.109(F16):FACS analysis (Day7)| Data analysis]]
+
| [[20.109(F16):Data analysis (Day7)| Analysis of sub-nuclear foci]]<br>[[Media:M1D7_Fa16_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M1D7_161007_marked.pdf| Prelab W/F]]
−
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M1D7| Homework due]]
+
| [[20.109(F16):Homework#Due_M1D7| Homework due]]
−
|
+
|--
 +
|
 +
|
 +
| <font color = 999999>T/W Oct 11/12</font color>
 +
|
 +
| <font color = 999999>Columbus day holiday</font color>
 +
| [[20.109(F16): M1 Data Summary| Data Summary draft due]] Wed, Oct 12 at 5 pm
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
Line 82: Line 92:
 
| 2
 
| 2
 
| 1
 
| 1
−
|
+
| R/F Oct 13/14
−
| [http://be.mit.edu/directory/noreen-lyell NLL]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D1 lecture slides.pdf|M2D1 lecture]]
−
|
+
| [[20.109(F16):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:Fa16_M2D1_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D1_161014_marked.pdf| Prelab W/F]]
−
|
+
| [[20.109(F16):Homework#Due_M2D1| Homework due]]<br>[[20.109(F16): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 15 at 10 pm
 +
|--
 +
| 2
 +
| 2
 +
| T/W Oct 18/19
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media:Fa16 M2D2 lecture slides.pdf|M2D2 lecture]]
 +
| [[20.109(F16):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:M2D2_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D2_161019_marked.pdf| Prelab W/F]]
 +
| [[20.109(F16):Homework#Due_M2D2| Homework due]]
 +
|--
 +
| 2
 +
| 3
 +
| R/F Oct 20/21
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D3 lecture slides.pdf|M2D3 lecture]]
 +
| [[20.109(F16):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:M2D3_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D3_161021_marked.pdf| Prelab W/F]]<br>[[Media:Fa16 M2D3 journal article discussion.pdf|Journal article discussion]]
 +
| [[20.109(F16):Homework#Due_M2D3| Homework due]]<br>[[20.109(F16): M1 Data Summary| Data Summary revision due]] Mon, Oct 24 at 5 pm
 +
|--
 +
| 2
 +
| 4
 +
| T/W Oct 25/26
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D4 lecture slides student.pdf|M2D4 lecture]]
 +
| [[20.109(F16):Journal club I (Day4)| Journal club I]]
 +
| <font color=#04B45F>'''Lab quiz'''</font color><br>[http://be20109f16.blogspot.com/ Blog post due] Tue, Oct 25 at 5pm
 +
|--
 +
| 2
 +
| 5
 +
| R/F Oct 27/28
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D5 lecture slides.pdf|M2D5 lecture]]
 +
| [[20.109(F16):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:M2D5 Pre-Lab TR.pdf| Prelab T/R]]<br>[[Media:Fa16 M2D5 EmilyClark 161027 pptx.pdf| Prelab W/F]]
 +
| [[20.109(F16):Homework#Due_M2D5| Homework due]]
 +
|--
 +
| 2
 +
| 6
 +
| T/W Nov 1/2
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D6 lecture slides.pdf|M2D6 lecture]]
 +
| [[20.109(F16):Journal club II (Day 6) | Journal club II]]
 +
| <font color=#04B45F>'''Lab quiz'''</font color><br>
 +
|--
 +
| 2
 +
| 7
 +
| R/F Nov 3/4
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D7 lecture slides.pdf|M2D7 lecture]]
 +
| [[20.109(F16):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:M2D7_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D7_161104_marked.pdf| Prelab W/F]]
 +
| [[20.109(F16):Homework#Due_M2D7| Homework due]]
 +
|--
 +
| 2
 +
| 8
 +
| T/W Nov 8/9
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa16 M2D8 lecture slides.pdf|M2D8 lecture]]
 +
| [[20.109(F16)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:M2D8_Fa16_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M2D8_161109_marked.pdf| Prelab W/F]]
 +
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M2D8| Homework due]]
 +
|--
 +
|
 +
|
 +
| <font color = 999999>R/F Nov 10/11</font color>
 +
|
 +
| <font color = 999999>Veterans' day holiday</font color>
 
|
 
|
 
|--
 
|--
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| 3
 
| 3
 
| 1
 
| 1
−
| R/F Nov 12/13 
+
| T/W Nov 15/16
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
−
| [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]]
+
| [[20.109(F16):Growth of phage materials (Day1)| Growth of phage materials]]<br>[[Media:Fa16 M3D1 prelab slides.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D1_161116_marked.pdf| Prelab W/F]]
−
| [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br> [[20.109(F16): Protein engineering report | Protein engineering report due]] Sun, Nov 15 at 5 pm‎
+
| [[20.109(F16):Homework#Wrapping_up_M2| Homework due]] <br>
−
|
+
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
−
| T/W Nov 17/18
+
| R/F Nov 17/18
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
−
| [[20.109(F16):Purify active materials (Day2)| Purify active materials]]
+
| [[20.109(F16):Purify active materials (Day2)| Purify active materials]]<br>[[Media:Fa16_M3D2_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D2_161118_marked.pdf| Prelab W/F]]
−
| [[20.109(F16):Homework#Due_M3D2| Homework due]]
+
| [[20.109(F16):Homework#Due_M3D2| Homework due]]<br>[[20.109(F16): Research article| Research article due]] Sun, Nov 20 at 5 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Mon, Nov 21 at 5pm
−
|
+
 
|--
 
|--
−
| 3
 
−
| 3
 
−
| R/F Nov 19/20
 
−
| [http://be.mit.edu/directory/angela-belcher AB] 
 
−
| [[20.109(F16):Cathode construction (Day3)| Cathode construction]]
 
−
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]]
 
 
|
 
|
−
|--
 
 
|
 
|
 +
| T/W Nov 22/23
 
|
 
|
−
| T/W Nov 24/25
+
| <font color = #FF0000>Lecture, but no laboratory</font color>
−
| [http://be.mit.edu/directory/angela-belcher AB]
+
−
| <font color = #FF0000>'''Lecture as scheduled, but no lab!'''</font color>
+
 
|  
 
|  
−
|
 
 
|--  
 
|--  
 
|
 
|
 
|
 
|
−
| <font color = 999999>R/F Nov 26/27</font color>
+
| <font color = 999999>R/F Nov 24/25</font color>
 
|
 
|
 
| <font color = 999999>Thanksgiving holiday</font color>
 
| <font color = 999999>Thanksgiving holiday</font color>
−
|  
+
|
 
|--  
 
|--  
 +
| 3
 +
| 3
 +
| T/W Nov 29/30
 +
| [http://be.mit.edu/directory/angela-belcher AB] 
 +
| [[20.109(F16):Cathode construction (Day3)| Cathode construction]]<br>[[Media:Fa16_M3D3_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D3_161130_marked.pdf| Prelab W/F]]
 +
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due_M3D3| Homework due]]
 +
|--
 
| 3
 
| 3
 
| 4
 
| 4
−
| T/W Dec 1/2  
+
| R/F Dec 1/2  
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
−
| [[20.109(F16):TEM (Day4)| TEM]]
+
| [[20.109(F16):TEM (Day4)| TEM]]<br>[[Media:Fa16_M3D4_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D4_161202_marked.pdf| Prelab W/F]]
 
| [[20.109(F16):Homework#Due_M3D4| Homework due]]
 
| [[20.109(F16):Homework#Due_M3D4| Homework due]]
−
|
 
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
−
| R/F Dec 3/4 
+
| T/W Dec 6/7 
 
| [http://be.mit.edu/directory/angela-belcher AB]   
 
| [http://be.mit.edu/directory/angela-belcher AB]   
−
| [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]]
+
| [[20.109(F16):Battery assembly and testing (Day5)| Battery assembly and testing]]<br>[[Media:Fa16_M3D5_LMM.pdf| Prelab T/R]]<br>[[Media:Fa16_M3D5_161207_marked.pdf| Prelab W/F]]
−
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]] <br> [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Thu/Fri, Dec 3/4 at 10 pm
+
| <font color=#04B45F>'''Lab quiz'''</font color><br>[[20.109(F16):Homework#Due (after) M3D5| Homework due]]<br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 7 at 10pm
−
|
+
 
|--
 
|--
 
| 3
 
| 3
 
| 6
 
| 6
−
| T/W Dec 8/9
+
| R/F Dec 8/9
 
|  
 
|  
 
| [[20.109(F16):Research proposal presentation| Research proposal presentations]]
 
| [[20.109(F16):Research proposal presentation| Research proposal presentations]]
−
| [[20.109(F16):Research proposal presentation| Research proposal presentation slides due]] Tue/Wed, Dec 8/9 at 1 pm
+
| [[20.109(F16):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 8/9 at 1 pm
−
|
+
|--
 +
| colspan="7" |
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
−
| R Dec 10
+
| T Dec 13
 
|  
 
|  
 
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color>
 
| <font color = #00BFFF>'''Feedback and celebratory lunch'''</font color>
−
|
+
| [[20.109(F16): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 12 at 10 pm <br>[http://be20109f16.blogspot.com/ Blog post due] Wed, Dec 14 at 10pm
−
|
+
 
|}
 
|}
 
</div>
 
</div>

Latest revision as of 18:39, 19 December 2016

20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 8/9 NLL
Orientation lecture 		Orientation
Prelab T/R
Prelab W/F
1 1 T/W Sept 13/14 BE Prepare microwell array and practice tissue culture
Prelab T/R
Prelab W/F 		Lab orientation quiz
Homework due
1 2 R/F Sept 15/16 BE Develop experiment to test loading variables and quantify growth rate
Prelab T/R
Prelab W/F 		Homework due
1 3 T/W Sept 20/21 BE Test role of biochemical factors in genomic stability
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
R/F Sept 22/23 BE Lecture, but no laboratory
Career fair student holiday
1 4 T/W Sept 27/28 NLL Query inter-individual variability in exposure susceptibility
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
1 5 R/F Sept 29/30 BE Develop approach for sub-nuclear visualization of DNA damage
Prelab T/R
Prelab W/F 		Homework due
1 6 T/W Oct 4/5 BE Query DNA repair capacity in tumor cells
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
1 7 R/F Oct 6/7 BE Analysis of sub-nuclear foci
Prelab T/R
Prelab W/F 		Homework due
T/W Oct 11/12 Columbus day holiday Data Summary draft due Wed, Oct 12 at 5 pm
2 1 R/F Oct 13/14 NLL
M2D1 lecture 		Complete in silico cloning
Prelab T/R
Prelab W/F 		Homework due
Mini-presentation due Sat, Oct 15 at 10 pm
2 2 T/W Oct 18/19 NLL
M2D2 lecture 		Design gRNA for CRISPRi
Prelab T/R
Prelab W/F 		Homework due
2 3 R/F Oct 20/21 NLL
M2D3 lecture 		Generate gRNA plasmid
Prelab T/R
Prelab W/F
Journal article discussion 		Homework due
Data Summary revision due Mon, Oct 24 at 5 pm
2 4 T/W Oct 25/26 NLL
M2D4 lecture 		Journal club I Lab quiz
Blog post due Tue, Oct 25 at 5pm
2 5 R/F Oct 27/28 NLL
M2D5 lecture 		Confirm gRNA sequence
Prelab T/R
Prelab W/F 		Homework due
2 6 T/W Nov 1/2 NLL
M2D6 lecture 		Journal club II Lab quiz
2 7 R/F Nov 3/4 NLL
M2D7 lecture 		Induce CRISPRi system
Prelab T/R
Prelab W/F 		Homework due
2 8 T/W Nov 8/9 NLL
M2D8 lecture 		Measure fermentation products
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
R/F Nov 10/11 Veterans' day holiday
3 1 T/W Nov 15/16 AB Growth of phage materials
Prelab T/R
Prelab W/F 		Homework due
3 2 R/F Nov 17/18 AB Purify active materials
Prelab T/R
Prelab W/F 		Homework due
Research article due Sun, Nov 20 at 5 pm
Blog post due Mon, Nov 21 at 5pm
T/W Nov 22/23 Lecture, but no laboratory
R/F Nov 24/25 Thanksgiving holiday
3 3 T/W Nov 29/30 AB Cathode construction
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
3 4 R/F Dec 1/2 AB TEM
Prelab T/R
Prelab W/F 		Homework due
3 5 T/W Dec 6/7 AB Battery assembly and testing
Prelab T/R
Prelab W/F 		Lab quiz
Homework due
Blog post due Wed, Dec 7 at 10pm
3 6 R/F Dec 8/9 Research proposal presentations Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm
T Dec 13 Feedback and celebratory lunch Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm
Blog post due Wed, Dec 14 at 10pm
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