Difference between revisions of "20.109(F15): TA's notes for module 3"
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MAXINE JONAS (Talk | contribs) (→Day 1) |
MAXINE JONAS (Talk | contribs) (→Day 2) |
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'''Between Day 1 and Day 2:''' | '''Between Day 1 and Day 2:''' | ||
| + | to be edited by George | ||
'''On the instructors' bench:''' | '''On the instructors' bench:''' | ||
*50 mL conical tubes | *50 mL conical tubes | ||
*15 mL conical tubes | *15 mL conical tubes | ||
| − | *tweezers | + | *tweezers (2 per team) |
| − | *TEM grids for practice | + | *TEM grids for practice (some) |
| + | *new TEM grids (2 per team) | ||
*sign-up sheet for location of samples in TEM holder | *sign-up sheet for location of samples in TEM holder | ||
| + | *100% ethanol (20 μL per team, so a couple of 1.5 mL eppendorf tubes suffice) | ||
'''On students' benches:''' | '''On students' benches:''' | ||
*Na:PO<sub>4</sub> | *Na:PO<sub>4</sub> | ||
| + | *M13 phage with(NH<sub>4</sub>)<sub>2</sub>Fe(SO<sub>4</sub>)<sub>2</sub> | ||
| + | quantities to be updated by George | ||
Revision as of 22:26, 17 November 2015
Day 1
The week preceding the lab:
- Make LB:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 L deionized water
- autoclave 30 minutes with stirbar.
On the instructors' bench:
- 50 mL conical tubes
- 25 mL pipets
- a couple of motorized pipet aids
- 2 mL eppendorf tubes
- TBS: 500 mL
- PEG/NaCl: 500 mL
- deionized water: 50 mL
- quartz cuvettes
On the students' bench:"
- bucket of ice
- 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage
- 250 mL erlenmeyer flask
By the scale:
- (NH4)2Fe(SO4)2
- stirbars
In 4 °C fridge:
- orbital shaker (speed 120)
Day 2
Between Day 1 and Day 2: to be edited by George
On the instructors' bench:
- 50 mL conical tubes
- 15 mL conical tubes
- tweezers (2 per team)
- TEM grids for practice (some)
- new TEM grids (2 per team)
- sign-up sheet for location of samples in TEM holder
- 100% ethanol (20 μL per team, so a couple of 1.5 mL eppendorf tubes suffice)
On students' benches:
- Na:PO4
- M13 phage with(NH4)2Fe(SO4)2
quantities to be updated by George
