Difference between revisions of "20.109(F15): TA's notes for module 3"
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MAXINE JONAS (Talk | contribs) (→Day 1) |
MAXINE JONAS (Talk | contribs) (→Day 1) |
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*250 mL erlenmeyer flask | *250 mL erlenmeyer flask | ||
| − | ''' | + | '''By the scale:''' |
* (NH<sub>4</sub>)<sub>2</sub>Fe(SO<sub>4</sub>)<sub>2</sub> | * (NH<sub>4</sub>)<sub>2</sub>Fe(SO<sub>4</sub>)<sub>2</sub> | ||
'''In 4 °C fridge:''' | '''In 4 °C fridge:''' | ||
*orbital shaker (speed 120) | *orbital shaker (speed 120) | ||
Revision as of 22:20, 12 November 2015
Day 1
The week preceding the lab:
- Make LB:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 L deionized water
- autoclave 30 minutes with stirbar.
On the instructors' bench:
- 50 mL conical tubes
- 25 mL pipets
- 2 mL eppendorf tubes
- TBS: 500 mL
- PEG/NaCl: 500 mL
- quartz cuvettes
On the students' bench:"
- bucket of ice
- 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage
- 250 mL erlenmeyer flask
By the scale:
- (NH4)2Fe(SO4)2
In 4 °C fridge:
- orbital shaker (speed 120)
