20.109(F15):Purify active materials (Day2)

Introduction

Protocols

Part 1: Demo of phage biomineralization reaction

Because the initial step of the biomineralization process is time-sensitive, George quenched your reactions afterdd ~12 h by adding 100 mL of 1 mM Na:PO₄. In this step, the phosphate precipitates the Fe(III) onto the p8 proteins of the M13 phage coat to generate nanowires. You will see a demo of this reaction in the next laboratory class.

Part 2: Collect active material (nanowires)

Part 3: Prepare TEM grid with nanowires

===Part 4: Dry active material (nanowires)===