Difference between revisions of "20.109(F15):Purify active materials (Day2)"
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Noreen Lyell (Talk | contribs) (Created page with "{{Template:20.109(F15)}} <div style="padding: 10px; width: 790px; border: 5px solid #0099FF;"> ==Introduction== ==Protocols== ===Part 1: Demo of phage biomineralization reac...") |
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===Part 1: Demo of phage biomineralization reaction=== | ===Part 1: Demo of phage biomineralization reaction=== | ||
| − | Because the initial step of the biomineralization process is time-sensitive, George quenched your reactions | + | Because the initial step of the biomineralization process is time-sensitive, George quenched your reactions after ~12 h by adding 100 mL of 1 mM Na:PO<sub>4</sub>. In this step, the phosphate precipitated the Fe(III) onto the p8 proteins of the M13 phage coat to generate the nanowires that will be used to construct the cathode of your battery. So you can see this reaction, George has prepared a small sample as a demo. |
===Part 2: Collect active material (nanowires)=== | ===Part 2: Collect active material (nanowires)=== | ||
| + | |||
| + | #Retrieve your flask with your Fe(III)-phage nanowires from the front bench. | ||
| + | #Gather the nanowires that adhered to the side of your flask by using a pipet to wash the culture liquid from your flask down the sides | ||
===Part 3: Prepare TEM grid with nanowires=== | ===Part 3: Prepare TEM grid with nanowires=== | ||
===Part 4: Dry active material (nanowires)=== | ===Part 4: Dry active material (nanowires)=== | ||
Revision as of 01:44, 14 November 2015
Contents
Introduction
Protocols
Part 1: Demo of phage biomineralization reaction
Because the initial step of the biomineralization process is time-sensitive, George quenched your reactions after ~12 h by adding 100 mL of 1 mM Na:PO4. In this step, the phosphate precipitated the Fe(III) onto the p8 proteins of the M13 phage coat to generate the nanowires that will be used to construct the cathode of your battery. So you can see this reaction, George has prepared a small sample as a demo.
Part 2: Collect active material (nanowires)
- Retrieve your flask with your Fe(III)-phage nanowires from the front bench.
- Gather the nanowires that adhered to the side of your flask by using a pipet to wash the culture liquid from your flask down the sides
