20.109(S22):Class data
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Contents
Module 1: Drug discovery
SDS-PAGE gel images
[SDS-PAGE dropbox folder https://www.dropbox.com/sh/1ggz8bk56diszpr/AAAFQLjMEgJcwdjrLg6jtRE5a?dl=0]
small molecule selections
T/R
Team | small molecule #1 (enter compound ID) | small molecule #2 (enter compound ID) | aggregation assay results |
TR Red | 83079118 | 69269200 | |
TR Orange | 95877382 | 83023303 | |
TR Yellow | 69269200 | 83079118 | |
TR Green | 69269200 | 83079118 | |
TR Blue | 69269200 | 83079118 | |
TR Teal | 69269200 | 83079118 | |
TR Pink | 69269200 | 83079118 | |
TR Purple | 69269200 | 83079118 | |
TR Grey | 69269200 | 83079118 | |
TR White | 69269200 | 83079118 |
W/F
Team | small molecule #1 (enter compound ID) | small molecule #2 (enter compound ID) | aggregation assay results |
WF Red | 69269200 | 83079118 | |
WF Orange | 83079118 | 69269200 | |
WF Yellow | 83079118 | 69269200 | |
WF Green | 83079118 | 83023303 | |
WF Blue | 69269200 | 83079118 | |
WF Pink | 83079118 | 69269200 | |
WF Purple | 69269200 | 83079118 | |
WF White | 69269200 | 83023303 |
Module 2: Metabolic engineering
sgRNA_target sequences
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Ethanol / Acetate Assay Results |
TR Red | ||||||
TR Orange | ||||||
TR Yellow | ||||||
TR Green | ||||||
TR Blue | ||||||
TR Teal | ||||||
TR Pink | ||||||
TR Purple | ||||||
TR Grey | ||||||
TR White |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Colorimetric Assay Results |
WF Red | ||||||
WF Orange | ||||||
WF Yellow | ||||||
WF Green | ||||||
WF Blue | ||||||
WF Teal | ||||||
WF Pink | ||||||
WF Purple | ||||||
WF Grey | ||||||
WF White |