20.109(F18): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
MODULE
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DAY
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DATE
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LECTURER
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LABORATORY EXPERIMENTS
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ASSIGNMENTS
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|
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R/F Sept 6/7
|
NLL Orientation lecture
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Orientation Prelab T/R Prelab W/F
|
|
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1
|
1
|
T/W Sept 11/12
|
BE
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Practice tissue culture and prepare microwell array Prelab T/R Prelab W/F
|
Laboratory orientation quiz Homework due
|
1
|
2
|
R/F Sept 13/14
|
BE
|
Design cell loading optimization experiment and research cell lines Prelab T/R Prelab W/F
|
Homework due
|
1
|
3
|
T/W Sept 18/19
|
BE
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Prepare and treat cells for genomic instability experiment Prelab T/R Prelab W/F
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Homework due
|
|
|
R/F Sept 20/21
|
BE
|
Lecture, but no laboratory Career fair student holiday
|
|
1
|
4
|
T/W Sept 25/26
|
Comm Lab
|
Complete genomic instability experiment and load cells for sub-nuclear foci assay Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
1
|
5
|
R/F Sept 27/28
|
BE
|
Analyze instability experiment data and treat cells for sub-nuclear foci assay Prelab T/R Prelab W/F
|
Homework due
|
1
|
6
|
T/W Oct 2/3
|
BE
|
Complete sub-nuclear foci assay Prelab T/R Prelab W/F
|
Homework due
|
1
|
7
|
R/F Oct 4/5
|
BE
|
Practice statistical analysis methods and complete data analysis Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
|
|
T/W Oct 9/10
|
|
Columbus day holiday
|
Data Summary draft due Wed, Oct 10 at 10 pm Blog post due Thurs, Oct 11 at 10 pm
|
|
2
|
1
|
R/F Oct 11/12
|
NLL M2D1 lecture
|
Complete in silico cloning Prelab T/R Prelab W/F
|
Homework due Mini-presentation due Sat, Oct 13 at 10 pm
|
2
|
2
|
T/W Oct 16/17
|
NLL M2D2 lecture
|
Design gRNA for CRISPRi Prelab T/R Prelab W/F
|
Homework due
|
2
|
3
|
R/F Oct 18/19
|
NLL Paper figures M2D3 slides
|
Generate gRNA plasmid Prelab T/R Prelab W/F
|
Homework due Data Summary revision due Sat, Oct 20 at 10 pm
|
2
|
4
|
T/W Oct 23/24
|
NLL M2D4 lecture
|
Journal club I
|
|
2
|
5
|
R/F Oct 25/26
|
NLL M2D5 lecture
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Confirm gRNA sequence Prelab T/R Prelab W/F
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Laboratory quiz Homework due
|
2
|
6
|
T/W Oct 30/31
|
NLL M2D6 lecture
|
Journal club II
|
|
2
|
7
|
R/F Nov 1/2
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NLL M2D7 lecture
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Induce CRISPRi system Prelab T/R Prelab W/F
|
Homework due Blog post due Fri, Nov 2 at 10 pm
|
2
|
8
|
T/W Nov 6/7
|
NLL M2D8 lecture
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Measure fermentation products Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
|
3
|
1
|
R/F Nov 8/9
|
AB
|
Grow phage-based active (cathode) material Prelab T/R
|
Homework due
|
|
|
T/W Nov 13/14
|
|
Veterans' day holiday
|
Research article due Mon, Nov 12 at 10 pm Blog post due Tue, Nov 13 at 10 pm
|
3
|
2
|
R/F Nov 15/16
|
AB
|
Purify phage-based active (cathode) material
|
Homework due
|
|
|
T/W Nov 20/21
|
AB
|
Lecture, but no laboratory
|
|
|
|
R/F Nov 22/23
|
|
Thanksgiving holiday
|
|
3
|
3
|
T/W Nov 27/28
|
AB
|
Construct cathode with phage-based active material
|
Laboratory quiz Homework due
|
3
|
4
|
R/F Nov/Dec 29/30
|
AB
|
Visualize phage nanowires using transmission electron microscopy
|
Homework due
|
3
|
5
|
T/W Dec 4/5
|
AB
|
Assemble and test batteries
|
Laboratory quiz Homework due
|
|
|
R/F Dec 6/7
|
|
Research proposal presentations
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Research proposal presentation due Thu/Fri, Dec 6/7 at 1 pm Blog post due Sat, Dec 8 at 10 pm
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|
|
|
T Dec 11
|
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Feedback and celebratory lunch
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Mini-report due Mon, Dec 10 at 10 pm
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