20.109(F16):Test comet chip loading variables (Day2)
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Contents
Introduction
experimental design...
Protocols
Part 1: Design experiment to optimize comet chip loading
You will design two experiments that test variables associated with loading cells into the microwells of your comet chip. In the first experiment you will consider the role of cell number. Specifically, how many cells should be added to each well to ensure the majority of the microwells are loaded? In addition, how many cells should be loaded into each microwell? The second experiment will interrogate the length of time needed for loading.
Experiment 1: Cell number
- How many cells should be loaded into each microwell?
- Consider the amount of DNA that is carried by a single mammalian cell and the detection limit provided by the SYBR gold DNA stain that will be used in your experiments.
- How many cells should be added to each well such that the desired number of cells are loaded into the microwells?
Experiment 2: Loading time
Part 2: Load comet chip
Reagents
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