OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.
T/R red team
name
|
OD562
|
standards
|
A
|
3.02
|
B
|
0.798
|
C
|
0.444
|
D
|
0.245
|
E
|
0.141
|
F
|
0.073
|
G
|
0.054
|
H
|
0.034
|
inverse pericam (IPC)
|
wild-type
|
0.177
|
mutant
|
0.201
|
W/F red team
name
|
OD562
|
standards
|
A
|
3.02
|
B
|
0.798
|
C
|
0.444
|
D
|
0.245
|
E
|
0.141
|
F
|
0.073
|
G
|
0.054
|
H
|
0.034
|
inverse pericam (IPC)
|
wild-type
|
0.177
|
mutant
|
0.201
|
W/F orange team
name
|
OD562
|
standards
|
A
|
2.970
|
B
|
0.672
|
C
|
0.337
|
D
|
0.174
|
E
|
0.086
|
F
|
0.037
|
G
|
0.018
|
H
|
0.000
|
inverse pericam (IPC)
|
wild-type
|
0.028
|
mutant
|
0.062
|
W/F blue team
name
|
OD562
|
standards
|
A
|
2.983
|
B
|
0.727
|
C
|
0.292
|
D
|
0.168
|
E
|
0.091
|
F
|
0.034
|
G
|
0.015
|
H
|
0.000
|
inverse pericam (IPC)
|
wild-type
|
0.049
|
mutant
|
0.027
|
W/F pink team
name
|
OD562
|
standards
|
A
|
2.943
|
B
|
0.699
|
C
|
0.341
|
D
|
0.166
|
E
|
0.101
|
F
|
0.038
|
G
|
0.017
|
H
|
0.000
|
inverse pericam (IPC)
|
wild-type
|
0.025
|
mutant
|
0.136
|
W/F purple team
name
|
OD562
|
standards
|
A
|
3.02
|
B
|
0.798
|
C
|
0.444
|
D
|
0.245
|
E
|
0.141
|
F
|
0.073
|
G
|
0.054
|
H
|
0.034
|
inverse pericam (IPC)
|
wild-type
|
0.177
|
mutant
|
0.201
|