20.109(S14): Spring 2014 schedule
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20.109(S14): Laboratory Fundamentals of Biological Engineering
Home
Schedule Spring 2014
Assignments
Module 1
Module 2
Module 3
MODULE
DAY
DATE
LECTURER
TA
LAB
NOTES
Tue/Wed Feb 4/5
ANS
Chris
Orientation
Introductory Lecture (PDF)
T/R Pre-Lab
W/F Pre-Lab
1
1
Thur/Fri Feb 6/7
JR
Chris
Microbial DNA extraction
lab orientation quiz
Lecture
L1 Notes
T/R Pre-Lab
W/F Pre-Lab
1
2
Tue/Wed Feb 11/12
JR
Chris
Diagnostic primer design
Lecture
L2 Notes
T/R Pre-lab
W/F Pre-Lab
1
3
Thur/Fri Feb 13/14
JR
Chris
16S PCR and paper discussion
lab quiz
Lecture
L3 Notes
T/R Pre-lab
W/F Pre-Lab
WAC lecture, talks
President's Day Feb 17
No lab Feb 18 or Feb 19
1
4
Thur/Fri Feb 20/21
JR
Chris
DNA cloning
L4 Notes
T/R Pre-lab
W/F Pre-Lab
WAC lecture, figures
1
5
Tue/Wed Feb 25/26
JR
Chris
DNA sequencing
lab quiz
Lecture
L5 notes
T/R Pre-lab
W/F Pre-lab
1
6
Thur/Fri Feb 27/28
JR
Chris
Journal club I
Lecture
L6 notes
1
7
Tue/Wed Mar 4/5
JR
Chris
Phylogenetic and primer analyses
Lecture
T/R Pre-lab
W/F Pre-Lab
1
8
Thur/Fri Mar 6/7
JR
Chris
Journal club II
lab quiz
Lecture
L7/8 notes
WAC lecture, abstracts
2
1
Tue/Wed Mar 11/12
LS
SV
Introduction to cell strains and plating
Lecture
T/R Prelab
W/F Pre-Lab
WAC lecture, IMRD
Draft Mod 1 Abstract and Summary Due WED 1 PM
2
2
Thur/Fri Mar 13/14
LS
SV
Begin Western protein analysis
Lecture
T/R Prelab
W/F Pre-Lab
2
3
Tue/Wed Mar 18/19
LS
SV
Choose system conditions and paper discussion
Mod 1 Memo Due
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
2
4
Thur/Fri Mar 20/21
LS
SV
Complete Western and prepare damaged DNA
Lecture
T/R Pre-lab
W/F Pre-Lab
Spring Break Mar 24-28
No lab all week
2
5
Tue/Wed Apr 1/2
LS
SV
Cell preparation for DNA repair assays
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
2
6
Thur/Fri Apr 3/4
LS
SV
DNA repair assays
Lecture
T/R Pre-Lab
W/F Pre-Lab
Final Mod 1 Abstract and Summary Due
2
7
Tue/Wed Apr 8/9
LS
SV
Data analysis
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
3
1
Thur/Fri Apr 10/11
ANS
RKA
Design experiment
Lecture
T/R Pre-Lab
W/F Pre-Lab
3
2
Tue/Wed Apr 15/16
ANS
RKA
Initiate cell culture
lab quiz
Lecture
3
3
Thur/Fri Apr 17/18
ANS
RKA
Testing cell viability
Lecture
Patriot's Day April 21-22
No lab Apr 22 or Apr 23
Mod 2 Report Due
3
4
Thu/Fri Apr 24/25
ANS
RKA
Preparing cells for analysis
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
Tue/Wed Apr 29/30
No lecture or lab today.
3
5
Thur/Fri May 1/2
ANS
RKA
Initiating transcript and protein assays
Lecture
T/R Pre-Lab
W/F Pre-Lab
3
6
Tue/Wed May 6/7
ANS
RKA
Transcript-level analysis
lab quiz
Lecture
T/R Pre-Lab
W/F Pre-Lab
3
7
Thur/Fri May 8/9
ANS
RKA
Protein-level and wrap-up analysis
Mod 3 Mini-report Due
T/R Pre-Lab
3
8
Tue/Wed May 13/14
ANS
RKA
Student presentations
Thur May 15
No lab session. Meet in
16-220
from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.
Category
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20.109ExportS14
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