20.109(S08): TA notes for module 1

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20.109(S08): Laboratory Fundamentals of Biological Engineering

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General notes

Plasmids used:

  • pCX-EGFP:
    • contains full-length EGFP gene
    • Amp resistant
    • f1 ori
    • SV40 ori
  • pCX-NNX:
    • a mock version of pCX-EGFP, contains no EGFP gene
    • Amp resistant
    • f1 ori
    • some altered restriction sites compared to pCX-EGFP, and SV40 ori deleted

Daily Notes

Day 1

Materials required:

  1. PCR Master Mix (2.5X), ~ 50 μL per group
  2. DI water, prep 100 μL aliquot per group
  3. 5 μL aliquots each of pCX-EGFP, D32N-fwd, D32N-rev
  4. 2 PCR tubes per group

Keep everything cold!

Day of Lab:

  • Lab orientation quiz (not a TA quiz) will be taken today.
  • Remember to freeze PCR products when they are ready.
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