20.109(S07): Growth of phage materials

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20.109: Laboratory Fundamentals of Biological Engineering

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Home        People        Schedule Spring 2007        Lab Basics        OWW Basics       
Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Introduction

Protocols

Part 1: M13.1

  • make competent cells
  • transform M13.1 and M13K07 into MG1655 and MDS60

Part 2: E4

infect M13 E4 into XL1-blues


For Next Time

  1. The primary assignment for this experimental module will be for you to develop a research proposal and present your idea to the class. For next time, please describe five recent findings that might define an interesting research question. You should hand in a 3-5 sentence description of each topic and list the reference that led you to each item. The topics you pick can be related to any aspect of the class, i.e. genome engineering, signal measurements, gene expression engineering or bio-material engineering. During lab next time, you and your partner will review the topics and narrow your choices, identifying one or perhaps two topics for further research.

Reagents

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