Search results

Create the page "Procedure" on this wiki!

• 20.109(S10):Characterize protein expression (Day6)
  ...lyse the bacteria, and run whole bacterial extracts on a protein gel. This procedure is called SDS-PAGE, for sodium docecyl sulfate-polyacrylamide gel electroph ...rocess three samples (the three +IPTG extracts) according to the following procedure. You should either time your spins with another group, or balance your tube
  14 KB (2,165 words) - 19:48, 28 July 2015
• 20.109(S10):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  7 KB (1,148 words) - 19:49, 28 July 2015
• 20.109(S10):RNA to DNA by RT-PCR (Day5)
  #Resuspend each sample in 44 μL of RNase-free water by the following procedure: ...deg;C while you prepare your samples. This preparation is required for the procedure to work optimally.
  7 KB (1,218 words) - 19:49, 28 July 2015
• 20.109(S10):Site-directed mutagenesis (Day2)
  ...smid DNA you used in your mutagenesis reaction, assuming that the miniprep procedure results in typical concentrations of 1 μg/μL.
  13 KB (2,131 words) - 19:49, 28 July 2015
• 20.109(S10):Bacterial amplification of DNA (Day3)
  ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
  11 KB (1,861 words) - 19:49, 28 July 2015
• 20.109(S10):Prepare expression system (Day4)
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  22 KB (3,701 words) - 19:49, 28 July 2015
• 20.109(S10):Preparing cells for analysis (Day4)
  ...ated to 50 °C while you prepare your samples. This is required for the procedure to work optimally.
  15 KB (2,534 words) - 19:49, 28 July 2015
• 20.109(S11):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others further back and to the side.'''
  7 KB (1,163 words) - 21:05, 28 July 2015
• 20.109(F12): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...rm what is commonly called a “mini-prep.” This term distinguishes the procedure from a “maxi-” or “large scale-prep” which involves a larger volume
  13 KB (2,328 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 7 FACS analysis
  ...just for counting and not for separating subpopulations of cells, then the procedure is called "flow cytometry," and this is what you will be doing today.
  5 KB (842 words) - 13:41, 29 July 2015
• 20.109(F12):Guidelines for working in the tissue culture facility
  #Bring only the items you need for a particular procedure into the hood to prevent cluttering your working space. Having a clear wor ...t the center or towards the back. Keep the objects needed for the current procedure within reach; keep the others in the back.
  10 KB (1,639 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  12 KB (1,926 words) - 13:41, 29 July 2015
• 20.109(S13):DNA cloning (Day4)
  ... and growing the bacteria in said antibiotic. (We’ll say more about that procedure below.) This method is especially suitable for cloning with the purpose of ...2, and Shannon's band was brighter, then Shannon and Agi would perform the procedure below with Shannon's PCR, but they wouldn't throw away Agi's PCR either.
  16 KB (2,679 words) - 13:47, 29 July 2015
• 20.109(S13):DNA sequencing and primer analysis (Day5)
  The procedure for DNA isolation at this scale is commonly termed "mini-prep," which disti Normally in 20.109 we do an in-house mini-prep procedure according to the steps above followed by ethanol precipitation. However, be
  16 KB (2,629 words) - 13:48, 29 July 2015
• 20.109(S13):Phylogenetic analysis (Day7)
  ...e an insert. However, note that there is a known problem with this cloning procedure wherein sometimes an incomplete vector (with no insert and also missing a c
  17 KB (2,753 words) - 13:48, 29 July 2015
• 20.109(S14):DNA cloning (Day4)
  ... and growing the bacteria in said antibiotic. (We’ll say more about that procedure below.) This method is especially suitable for cloning with the purpose of ...andled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 10<
  15 KB (2,521 words) - 13:58, 29 July 2015
• 20.109(S14):DNA sequencing (Day5)
  The procedure for DNA isolation at this scale is commonly termed "mini-prep," which disti Normally in 20.109 we do an in-house mini-prep procedure according to the steps above followed by ethanol precipitation. However, be
  17 KB (2,778 words) - 13:58, 29 July 2015
• 20.109(S14):DNA repair assays(Day6)
  ...just for counting and not for separating subpopulations of cells, then the procedure is called flow cytometry, while cell sorting or isolation is called FACS. H #*Each T25 flask of cells will be trypsinized according to the M2D1 procedure.
  17 KB (2,912 words) - 13:59, 29 July 2015
• 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
  ... to confirm the appropriate mutation was incorporated. The transformation procedure will be as follows:
  28 KB (4,525 words) - 14:45, 12 November 2015
• 20.109(F15):Bacterial amplification of DNA (Day3)
  ...smid, you might increase the amount of DNA used during the transformation procedure at the end of lab.
  13 KB (2,207 words) - 11:40, 4 September 2015