Difference between revisions of "Optical Microscopy: Part 3 Report Outline"
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<li>Report the resolution you measured. Make sure to include N and a measure of uncertainty.</li> | <li>Report the resolution you measured. Make sure to include N and a measure of uncertainty.</li> | ||
<li>Show sample Gaussian fits.</li> | <li>Show sample Gaussian fits.</li> | ||
− | <li> | + | <li>Explain the Matlab algorithm used for data analysis.</li> |
</ul> | </ul> | ||
<li>Discussion</li> | <li>Discussion</li> | ||
<ul> | <ul> | ||
<li>Compare the measured value to the theoretical value.</li> | <li>Compare the measured value to the theoretical value.</li> | ||
+ | <li>Include a thorough discussion of error sources.</li> | ||
</ul> | </ul> | ||
</ol> | </ol> |
Revision as of 16:03, 5 March 2014
- Resolution
- Procedure
- Document the samples you used and how you captured images (camera settings, software used, etc…)
- Data
- Include an image of the PSF sample with the beads used for resolution measurement indicated.
- Analysis and Results
- Report the resolution you measured. Make sure to include N and a measure of uncertainty.
- Show sample Gaussian fits.
- Explain the Matlab algorithm used for data analysis.
- Discussion
- Compare the measured value to the theoretical value.
- Include a thorough discussion of error sources.
- Procedure
- Stability
- Procedure
- Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
- Data
- Show an example frame from the stability movie.
- Provide X-Y plots of difference tracks for pairs of fixed particles.
- Plot MSD versus time interval τ for sum and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
- Analysis and Results
- Provide a bullet point outline of data analysis methodology.
- Include a thorough discussion of error sources.
- Discussion
- What are the benefits and drawbacks of differential tracking?
- Procedure