Difference between revisions of "20.109(S22):Class data"
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Revision as of 18:55, 7 March 2022
Contents
Module 1: Drug discovery
SDS-PAGE gel images
small molecule selections
T/R
W/F
Team | small molecule #1 (enter compound ID) | small molecule #2 (enter compound ID) | aggregation assay results | |
WF Red | 69269200 | 83079118 | https://mitprod-my.sharepoint.com/:x:/g/personal/mrowlett_mit_edu/EXXtvBLjG9BLtZajiE1PqbUBBkSUVd5xolqzA2KO1u1ADg?e=JjooPh | |
WF Orange | 83079118 | 69269200 | https://www.dropbox.com/s/8ksmmtml3e673m5/WFOrange%20Aggregation%20Assay%20Data.xlsx?dl=0 | |
WF Yellow | 83079118 | 69269200 | ||
WF Green | 83079118 | 83023303 | ||
WF Blue | 69269200 | 83079118 | https://www.dropbox.com/scl/fi/zogoszln7ydo10em8b196/20220218_agg_assay_blue-83079118.xlsx?dl=0&rlkey=glixenu1uqfo6xa5t1fmvmi08 | |
WF Pink | 83079118 | 69269200 | ||
WF Purple | 69269200 | 83079118 | https://docs.google.com/spreadsheets/d/1WXBr83b12vb83pYv1bqTUPl5VGFIDap9uOjESwOH0tQ/edit?usp=sharing | |
WF White | 69269200 | 83023303
https://www.dropbox.com/s/c2g5513td5fepee/Aggregation%20Data.xlsx?dl=0 |
Aggregation Results
[T/R Aggregation dropbox folder]
Localization Results
Module 2: Metabolic engineering
sgRNA_target sequences
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Ethanol / Acetate Assay Results |
TR Red | ||||||
TR Orange | ||||||
TR Yellow | ||||||
TR Green | ||||||
TR Blue | ||||||
TR Teal | ||||||
TR Pink | ||||||
TR Purple | ||||||
TR Grey | ||||||
TR White |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target coding or non-coding strand | Colorimetric Assay Results |
WF Red | ||||||
WF Orange | ||||||
WF Yellow | ||||||
WF Green | ||||||
WF Blue | ||||||
WF Teal | ||||||
WF Pink | ||||||
WF Purple | ||||||
WF Grey | ||||||
WF White |