Difference between revisions of "Spring 11:QRT-PCR weekly reports"

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(3/18/11)
Line 5: Line 5:
 
Next Week:
 
Next Week:
 
* research and order parts needed for heated lid
 
* research and order parts needed for heated lid
* figure out why the sginal amplification is so low
 
 
* verify optical setup with mock system (blue LED + ND filter)
 
* verify optical setup with mock system (blue LED + ND filter)
 +
* verify current optical and heating setup by talking a DNA melting curve
 +
* start thinking about the QRT-PCR analysis code
  
 
Risks and Issues:
 
Risks and Issues:
 
* ordered parts for heated lid may not work, or may not come in for a while
 
* ordered parts for heated lid may not work, or may not come in for a while
 +
* PCR may not work even with heated lid - will need to think of other ways to fix this problem.

Revision as of 18:31, 19 March 2011

3/18/11

This Week:

  • Achieved photodiode signal with new fiber optic setup

Next Week:

  • research and order parts needed for heated lid
  • verify optical setup with mock system (blue LED + ND filter)
  • verify current optical and heating setup by talking a DNA melting curve
  • start thinking about the QRT-PCR analysis code

Risks and Issues:

  • ordered parts for heated lid may not work, or may not come in for a while
  • PCR may not work even with heated lid - will need to think of other ways to fix this problem.