Difference between revisions of "20.109(F16):Module 2"
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==Lab links: day by day== | ==Lab links: day by day== | ||
− | [[20.109(F16):Complete in silico cloning (Day1)| | + | [[20.109(F16):Complete in silico cloning (Day1)| M2D1: Complete in silico cloning]]<br> |
− | [[20.109(F16):Design gRNA for CRISPRi (Day2)| | + | [[20.109(F16):Design gRNA for CRISPRi (Day2)| M2D2: Design gRNA for CRISPRi]]<br> |
− | [[20.109(F16):Generate gRNA plasmid (Day3)| | + | [[20.109(F16):Generate gRNA plasmid (Day3)| M2D3: Generate gRNA plasmid]]<br> |
− | [[20.109(F16):Journal club I (Day4)| | + | [[20.109(F16):Journal club I (Day4)| M2D4: Journal club I]]<br> |
− | [[20.109(F16):Confirm gRNA sequence (Day5)| | + | [[20.109(F16):Confirm gRNA sequence (Day5)| M2D5: Confirm gRNA sequence]]<br> |
− | [[20.109(F16):Journal club II (Day 6) | | + | [[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> |
− | [[20.109(F16):Induce CRISPRi system (Day7)| | + | [[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> |
− | [[20.109(F16):Measure fermentation products (Day8)| | + | [[20.109(F16):Measure fermentation products (Day8)| M2D8: Measure fermentation products]]<br> |
==Assignments== | ==Assignments== |
Revision as of 15:24, 28 July 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
paper for CRISPRi system