Difference between revisions of "20.109(S11): TA notes for module 2"
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===Day 1=== | ===Day 1=== | ||
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| + | NOT YET COMPLETE - SEE ALSO GOOGLE DOC | ||
'''Materials required:''' | '''Materials required:''' | ||
| − | '''Day of Lab ( | + | * Two days before lab, streak out the following strains: |
| + | ** ABX(8?) = pEDL3/pCph8/pPLPCB, on Amp/Cam/Kan plate | ||
| + | ** ABX(22?) = pED-IPTG-INS, on Amp plate | ||
| + | * One day before lab, prepare O/N cultures of same | ||
| + | **AB8? is for edge detection plates | ||
| + | **AB22? is for first liquid culture experiment | ||
| + | |||
| + | Below are at set up at teaching bench: | ||
| + | |||
| + | * 2 boxes of cuvettes | ||
| + | |||
| + | '''Day of Lab (T/R):''' | ||
| + | |||
| + | * Prepare supplemented LB medium (30' autoclave, 30' or 60' for pressure to go down in large or small autoclave, respectively) and cool in a 42 °C water bath for at least 1 hour | ||
'''After Lab''' | '''After Lab''' | ||
Revision as of 19:09, 6 March 2011
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General notes
Key preparation:
Scheme:
Day-by-day
Day 1
NOT YET COMPLETE - SEE ALSO GOOGLE DOC
Materials required:
- Two days before lab, streak out the following strains:
- ABX(8?) = pEDL3/pCph8/pPLPCB, on Amp/Cam/Kan plate
- ABX(22?) = pED-IPTG-INS, on Amp plate
- One day before lab, prepare O/N cultures of same
- AB8? is for edge detection plates
- AB22? is for first liquid culture experiment
Below are at set up at teaching bench:
- 2 boxes of cuvettes
Day of Lab (T/R):
- Prepare supplemented LB medium (30' autoclave, 30' or 60' for pressure to go down in large or small autoclave, respectively) and cool in a 42 °C water bath for at least 1 hour
After Lab
How it went:
Day 2
Etc.
Special materials
Cell strains
- NB399 = JW3367c (EnvZ-, lacZ-, Kan^R removed)
- NB435/AB? = pEDL3, pCph8, pPL-PCB (full ED system)
- NB? = light to lacZ only
- AB? = pED-IPTG-110
- AB? = pED-IPTG-112
- AB? = pJT104 (full ED system less AHL)
