Difference between revisions of "20.109(S07): Measuring calcium in vivo"
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==Introduction== | ==Introduction== | ||
| − | Microscopy can reveal aspects of cellular structures that other techniques cannot address. Information about a cell's shape, its cellular compartments and the components of those compartments are all routinely collected thanks to laboratory microscopes no different than the one you'll use today. Furthermore, as research to improve microscopy techniques continues, breakthroughs are reported that improve detection limits imposed by light's diffraction [http://www.hhmi.org/news/palm20060810.html for example the PALM microscopy] | + | Microscopy can reveal aspects of cellular structures that other techniques cannot address. Information about a cell's shape, its cellular compartments and the components of those compartments are all routinely collected thanks to laboratory microscopes no different than the one you'll use today. Furthermore, as research to improve microscopy techniques continues, breakthroughs are reported that improve [http://www.microscopyu.com/articles/digitalimaging/drentdigital.html digital image analysis] and that increase detection limits imposed by light's diffraction [http://www.hhmi.org/news/palm20060810.html , for example the recent description of PALM microscopy]. |
==Protocols== | ==Protocols== | ||
Revision as of 11:10, 28 December 2006
Introduction
Microscopy can reveal aspects of cellular structures that other techniques cannot address. Information about a cell's shape, its cellular compartments and the components of those compartments are all routinely collected thanks to laboratory microscopes no different than the one you'll use today. Furthermore, as research to improve microscopy techniques continues, breakthroughs are reported that improve digital image analysis and that increase detection limits imposed by light's diffraction , for example the recent description of PALM microscopy.
Protocols
Microscopy
- visualize cells vs controls
- modulate Ca++ with ionophores/chelators
DONE!
