Difference between revisions of "20.109(F11): Mod 1 Day 4 DNA ligations and bacterial transformations"
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(→Part 4: Bacterial Transformations) |
(→Part 4: Bacterial Transformations) |
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[[Image:Mod1 3 sche bact.jpg|thumb|right|400px|'''Bacterial transformation''']] | [[Image:Mod1 3 sche bact.jpg|thumb|right|400px|'''Bacterial transformation''']] | ||
− | If all goes well, your ligation reactions will generate your desired construct: the pCX-NXX backbone carrying the EGFP gene lacking the first 32 amino acids. You will transform this product into bacteria. During | + | If all goes well, your ligation reactions will generate your desired construct: the pCX-NXX backbone carrying the EGFP gene lacking the first 32 amino acids. You will transform this product into bacteria. During |