Difference between revisions of "20.109(S11): TA notes for module 2"
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==Special materials== | ==Special materials== | ||
| + | |||
| + | ===Cell strains=== | ||
| + | |||
| + | *NB399 = JW3367c (EnvZ-, lacZ-, Kan^R removed) | ||
| + | *NB435/AB? = pEDL3, pCph8, pPL-PCB (full ED system) | ||
| + | *NB? = light to lacZ only | ||
| + | *AB? = pED-IPTG-110 | ||
| + | *AB? = pED-IPTG-112 | ||
| + | *AB? = pJT104 (full ED system less AHL) | ||
Revision as of 14:52, 16 December 2010
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General notes
Key preparation:
Scheme:
Day-by-day
Day 1
Materials required:
Day of Lab (X/Y):
After Lab
How it went:
Day 2
Etc.
Special materials
Cell strains
- NB399 = JW3367c (EnvZ-, lacZ-, Kan^R removed)
- NB435/AB? = pEDL3, pCph8, pPL-PCB (full ED system)
- NB? = light to lacZ only
- AB? = pED-IPTG-110
- AB? = pED-IPTG-112
- AB? = pJT104 (full ED system less AHL)
