Difference between revisions of "20.109(S23):Class data"
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<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | <div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | ||
{{Template:20.109(S23)}} | {{Template:20.109(S23)}} | ||
− | |||
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====W/F==== | ====W/F==== | ||
− | Dropbox Link : https://www.dropbox.com/sh/ | + | Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0 |
===BCA=== | ===BCA=== | ||
====W/F==== | ====W/F==== | ||
[[Media: Sp23 W F BCA.xlsx]] | [[Media: Sp23 W F BCA.xlsx]] | ||
+ | |||
+ | ===Western Blot=== | ||
+ | ====W/F==== | ||
+ | |||
+ | Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf | ||
+ | |||
+ | ===SMM=== | ||
+ | |||
+ | [[Media:Screen results.csv | SMM results]] | ||
==Module 2: Protein engineering== | ==Module 2: Protein engineering== | ||
Line 35: | Line 43: | ||
|'''Forward primer''' | |'''Forward primer''' | ||
|'''Reverse primer''' | |'''Reverse primer''' | ||
− | |||
|- | |- | ||
|TR red | |TR red | ||
− | | | + | | Y276C |
− | | | + | | TGGCAGATGTgTATTAACACTG |
− | | | + | | AGCGTCACTGAACTTTTTC |
|- | |- | ||
|TR orange | |TR orange | ||
− | | | + | | Y276C |
− | | | + | | TTGGCAGATGtgtATTAACACTG |
− | | | + | | GCGTCACTGAACTTTTTC |
|- | |- | ||
|TR yellow | |TR yellow | ||
− | | | + | | Y276C |
− | | | + | | GGCAGATGTAcATTAACACTG |
− | | | + | | AAGCGTCACTGAACTTTTTC |
|- | |- | ||
|TR green | |TR green | ||
− | | | + | | Y352C |
− | | | + | | GATTGATTGGtgtGCTGATATTATTG |
− | | | + | | ATCTTTCTCCCTGTCGAC |
|- | |- | ||
|TR blue | |TR blue | ||
− | | | + | | Y276C |
− | | | + | | TGGCAGATGTgTATTAACACTG |
− | | | + | | AGCGTCACTGAACTTTTTC |
|- | |- | ||
|TR pink | |TR pink | ||
− | | | + | | D354C |
− | | | + | | TTGGTATGCTtgtATTATTGGTACTGG |
− | | | + | | TCAATCATCTTTCTCCCTG |
|- | |- | ||
|TR purple | |TR purple | ||
− | | | + | | Y276C |
− | | | + | | TGGCAGATGTgTATTAACACTG |
− | | | + | | AGCGTCACTGAACTTTTTC |
|} | |} | ||
+ | |||
+ | '''T/R Sequencing [[https://www.dropbox.com/sh/tt7s2bmbuwzag23/AADj_lUJ3AirAlREGh9OqL1Za?dl=0 here]]''' | ||
====W/F==== | ====W/F==== | ||
Line 84: | Line 93: | ||
|- | |- | ||
|WF red | |WF red | ||
− | | | + | | V281C |
− | | | + | | TAACACTGCAtgTGCAGTTTCCCTTC |
− | | | + | | ATATACATCTGCCAAGCG |
|- | |- | ||
|WF orange | |WF orange | ||
− | | | + | | I349C |
− | | | + | | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG |
− | | | + | | CCTGTCGACCTTTTTTTAG |
|- | |- | ||
|WF yellow | |WF yellow | ||
− | | | + | | R405C |
− | | | + | | TTTCGTTTTGtgtGAAGTGTACTTCCG |
− | | | + | | CCGTCCAAAAACCCAATTAAAC |
|- | |- | ||
|WF green | |WF green | ||
− | | | + | | D271C |
− | | | + | | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) |
− | | | + | | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
|- | |- | ||
|WF blue | |WF blue | ||
− | | | + | | Y408C |
| | | | ||
| | | | ||
|- | |- | ||
|WF pink | |WF pink | ||
− | | | + | | D400C |
− | | | + | | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) |
− | | | + | | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
|- | |- | ||
|WF purple | |WF purple | ||
− | | | + | | D350C |
− | | | + | | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC |
− | | | + | | CTCCCTGTCGACCTTTTTTTAG |
|} | |} | ||
+ | |||
+ | '''WF Sequencing [https://www.dropbox.com/sh/jtmnkq24jqdbbsa/AABGRU3EhmCNecalRfzSNkwna?dl=0 here]''' | ||
+ | |||
+ | ===Immunofluorescence Data=== | ||
+ | Pilot Immunofluorescence Data [[https://www.dropbox.com/sh/lul2lkwiv4n5s2c/AADwI02c7chXUc1wiuJrNIvma?dl=0]] | ||
===ICP-OES data=== | ===ICP-OES data=== | ||
Line 125: | Line 139: | ||
{| border=1px | {| border=1px | ||
|'''Team''' | |'''Team''' | ||
− | |''' | + | |'''Fe (ppm)/ Wavelength''' |
− | |''' | + | |'''Cd (ppm)/ Wavelength''' |
− | |''' | + | |'''O.D.''' |
|- | |- | ||
|TR red | |TR red | ||
− | | | + | | 2.62 ppm / 240.489 nm |
− | | | + | | 7.58 ppm / 361.287 nm |
− | | | + | | 1.889 |
|- | |- | ||
|TR orange | |TR orange | ||
− | | | + | | 4.29 ppm / 261.382 nm |
− | | | + | | 11.16 ppm / 326.105 nm |
− | | | + | | 1.566 |
|- | |- | ||
|TR yellow | |TR yellow | ||
− | | | + | | 3.70 ppm / 261.382 nm |
− | | | + | | 11.35 ppm / 346.766 nm |
− | | | + | | 1.529 |
|- | |- | ||
|TR green | |TR green | ||
− | | | + | | 4.58 ppm / 238.863 nm |
− | | | + | | 11.50 ppm / 479.991 nm |
− | | | + | | 1.495 |
|- | |- | ||
|TR blue | |TR blue | ||
− | | | + | | 4.66 ppm / 259.837 nm |
− | | | + | | 11.52 ppm /479.999 nm |
− | | | + | | 1.546 |
|- | |- | ||
|TR pink | |TR pink | ||
+ | | 4.03ppm / 238.863nm | ||
+ | | 10.52ppm / 232.929nm | ||
+ | | 1.581 | ||
+ | |- | ||
+ | |TR purple | ||
+ | | 4.19 ppm / 238.204 nm | ||
+ | | 10.90 ppm / 223.986 nm | ||
+ | | 1.458 | ||
+ | |- | ||
+ | |TR WT | ||
| | | | ||
| | | | ||
− | | | + | | 1 |
|- | |- | ||
− | |TR | + | |TR Fet4 |
− | + | ||
| | | | ||
| | | | ||
+ | | 1.28 | ||
|} | |} | ||
Line 170: | Line 194: | ||
{| border=1px | {| border=1px | ||
|'''Team''' | |'''Team''' | ||
− | |''' | + | |'''Fe (ppm)/ Wavelength''' |
− | |''' | + | |'''Cd (ppm)/ Wavelength''' |
− | |''' | + | |'''O.D.''' |
|- | |- | ||
|WF red | |WF red | ||
− | | | + | | 4.09 / 238.863 |
− | | | + | | 10.99 /213.439 |
− | | | + | | 1.486 |
|- | |- | ||
|WF orange | |WF orange | ||
− | | | + | | 3.73 / 238.204 |
− | | | + | | 11.63 / 226.502 |
− | | | + | | 1.427 |
|- | |- | ||
|WF yellow | |WF yellow | ||
− | | | + | | 4.22 / 238.204 |
− | | | + | | 10.47 / 361.287 |
− | | | + | | 1.421 |
|- | |- | ||
|WF green | |WF green | ||
Line 196: | Line 220: | ||
|- | |- | ||
|WF blue | |WF blue | ||
− | | | + | | 5.17 / 238.863 |
− | | | + | | 12.58 / 214.439 |
− | | | + | | 1.322 |
|- | |- | ||
|WF pink | |WF pink | ||
+ | | 4.78 / 261.382 | ||
+ | | 10.89 / 361.287 | ||
+ | | 1.486 | ||
+ | |- | ||
+ | |WF purple | ||
+ | | 4.52 / 261.382 | ||
+ | | 11.52 / 361.287 | ||
+ | | 1.205 | ||
+ | |- | ||
+ | |WF WT | ||
| | | | ||
| | | | ||
− | | | + | | 1.18 |
|- | |- | ||
− | |WF | + | |WF Fet4 |
− | + | ||
| | | | ||
| | | | ||
+ | | 1.4 | ||
|} | |} | ||
===Cytotoxicity data=== | ===Cytotoxicity data=== | ||
[[https://www.dropbox.com/sh/rjhotqgpy2v37se/AACOnsHoEBZMNvQ7DRd2D5Yma?dl=0 TR section]] | [[https://www.dropbox.com/sh/rjhotqgpy2v37se/AACOnsHoEBZMNvQ7DRd2D5Yma?dl=0 TR section]] | ||
− | [[https://www.dropbox.com/ | + | [[https://www.dropbox.com/s/u0ydemw0472ekz2/WF.xlsx?dl=0 WF section]] |
+ | |||
+ | 1) Untransformed No Cadmium OD - 1.5 | ||
+ | 2) Untransformed Cadmium OD - 1.4 | ||
+ | 3) FET4 No Cadmium OD - 1.44 | ||
+ | 4) FET4 Cadmium OD - 1.44 | ||
+ | 5) Purple No Cadmium 1.733 | ||
+ | 6) Purple Cadmium 1.778 |
Latest revision as of 17:59, 12 May 2023
Contents
Module 1: Drug discovery
SDS-PAGE
T/R
Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg
W/F
Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0
BCA
W/F
Western Blot
W/F
Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf
SMM
Module 2: Protein engineering
Mutagenesis
T/R
Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
TR red | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
TR orange | Y276C | TTGGCAGATGtgtATTAACACTG | GCGTCACTGAACTTTTTC |
TR yellow | Y276C | GGCAGATGTAcATTAACACTG | AAGCGTCACTGAACTTTTTC |
TR green | Y352C | GATTGATTGGtgtGCTGATATTATTG | ATCTTTCTCCCTGTCGAC |
TR blue | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
TR pink | D354C | TTGGTATGCTtgtATTATTGGTACTGG | TCAATCATCTTTCTCCCTG |
TR purple | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
T/R Sequencing [here]
W/F
Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
WF red | V281C | TAACACTGCAtgTGCAGTTTCCCTTC | ATATACATCTGCCAAGCG |
WF orange | I349C | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | CCTGTCGACCTTTTTTTAG |
WF yellow | R405C | TTTCGTTTTGtgtGAAGTGTACTTCCG | CCGTCCAAAAACCCAATTAAAC |
WF green | D271C | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
WF blue | Y408C | ||
WF pink | D400C | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
WF purple | D350C | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | CTCCCTGTCGACCTTTTTTTAG |
WF Sequencing here
Immunofluorescence Data
Pilot Immunofluorescence Data [[1]]
ICP-OES data
T/R
Raw ICP-OES data [here]
Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
TR red | 2.62 ppm / 240.489 nm | 7.58 ppm / 361.287 nm | 1.889 |
TR orange | 4.29 ppm / 261.382 nm | 11.16 ppm / 326.105 nm | 1.566 |
TR yellow | 3.70 ppm / 261.382 nm | 11.35 ppm / 346.766 nm | 1.529 |
TR green | 4.58 ppm / 238.863 nm | 11.50 ppm / 479.991 nm | 1.495 |
TR blue | 4.66 ppm / 259.837 nm | 11.52 ppm /479.999 nm | 1.546 |
TR pink | 4.03ppm / 238.863nm | 10.52ppm / 232.929nm | 1.581 |
TR purple | 4.19 ppm / 238.204 nm | 10.90 ppm / 223.986 nm | 1.458 |
TR WT | 1 | ||
TR Fet4 | 1.28 |
W/F
Raw ICP-OES data [here]
Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
WF red | 4.09 / 238.863 | 10.99 /213.439 | 1.486 |
WF orange | 3.73 / 238.204 | 11.63 / 226.502 | 1.427 |
WF yellow | 4.22 / 238.204 | 10.47 / 361.287 | 1.421 |
WF green | |||
WF blue | 5.17 / 238.863 | 12.58 / 214.439 | 1.322 |
WF pink | 4.78 / 261.382 | 10.89 / 361.287 | 1.486 |
WF purple | 4.52 / 261.382 | 11.52 / 361.287 | 1.205 |
WF WT | 1.18 | ||
WF Fet4 | 1.4 |
Cytotoxicity data
[TR section] [WF section]
1) Untransformed No Cadmium OD - 1.5 2) Untransformed Cadmium OD - 1.4 3) FET4 No Cadmium OD - 1.44 4) FET4 Cadmium OD - 1.44 5) Purple No Cadmium 1.733
6) Purple Cadmium 1.778