Difference between revisions of "20.109(F17): Fall 2017 Schedule"
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{{Template:20.109(F17)}} | {{Template:20.109(F17)}} | ||
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<font color=#31B404>'''SCHEDULE DETAILS:'''</font color><br> | <font color=#31B404>'''SCHEDULE DETAILS:'''</font color><br> | ||
− | <font color=#31B404>'''Lecture times:'''</font color> T/R 11-12 ( | + | <font color=#31B404>'''Lecture times:'''</font color> T/R 11-12 (16-220) <br> |
<font color=#31B404>'''Laboratory section times:'''</font color> T/R 1-5 or W/F 1-5 (56-322)<br> | <font color=#31B404>'''Laboratory section times:'''</font color> T/R 1-5 or W/F 1-5 (56-322)<br> | ||
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| | | | ||
| R/F Sept 7/8 | | R/F Sept 7/8 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Orientation lecture]] | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 Orientation lecture.pdf| Orientation lecture]] |
− | | [[20.109(F17):Lab tour | Orientation]]<br> | + | | [[20.109(F17):Lab tour | Orientation]]<br>[[Media:Fa17_M0D0_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 Orientation NLL marked.pdf| Prelab W/F]] |
| | | | ||
|-- | |-- | ||
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| T/W Sept 12/13 | | T/W Sept 12/13 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F17):Prepare comet chips (Day1) | Prepare microwell array and practice tissue culture]]<br> | + | | [[20.109(F17):Prepare comet chips (Day1) | Prepare microwell array and practice tissue culture]]<br>[[Media:M1D1_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D1 NLL marked.pdf| Prelab W/F]] |
− | | <font color=# | + | | <font color=#0174DF>'''Laboratory orientation quiz'''</font color><br>[[20.109(F17):Homework#Due_M1D1|Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 2 | | 2 | ||
| R/F Sept 14/15 | | R/F Sept 14/15 | ||
− | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/noreen-lyell NLL] |
− | | [[20.109(F17): | + | | [[20.109(F17):Develop experiment to optimize loading variables (Day2)| Develop experiment to optimize cell loading]]<br>[[Media:M1D2_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D2 NLL marked.pdf| Prelab W/F]] |
− | |[[Homework due]] | + | | [[20.109(F17):Homework#Due_M1D2|Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
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| T/W Sept 19/20 | | T/W Sept 19/20 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F17): | + | | [[20.109(F17):Evaluate cell loading results (Day3)| Evaluate cell loading results]]<br>[[Media:M1D3_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D3 NLL marked.pdf| Prelab W/F]] |
− | | | + | | [[20.109(F17):Homework#Due_M1D3|Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
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| R/F Sept 21/22 | | R/F Sept 21/22 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F17): | + | | [[20.109(F17):Test role of biochemical factors in genomic stability (Day4)| Test role of biochemical factors in genomic stability]]<br>[[Media:M1D4_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D4 NLL marked.pdf| Prelab W/F]] |
− | | [[Homework due]] | + | | [[20.109(F17):Homework#Due_M1D4|Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 5 | | 5 | ||
| T/W Sept 26/27 | | T/W Sept 26/27 | ||
− | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/bevin-p-engelward BE] |
− | | [[20.109(F17): | + | | [[20.109(F17):Complete biochemical experiment (Day5)| Complete biochemical experiment and apply chemical treatments for sub-nuclear foci assay]]<br>[[Media:M1D5_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D5 NLL marked.pdf| Prelab W/F]] |
− | | <font color=# | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M1D5|Homework due]] |
|-- | |-- | ||
| | | | ||
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| R/F Sept 28/29 | | R/F Sept 28/29 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | Lecture, but no laboratory</font color><br><font color = 999999>Career fair student holiday</font color><br> | + | | <font color = #31B404>'''Lecture, but no laboratory'''</font color><br><font color = 999999>Career fair student holiday</font color><br> |
| | | | ||
|-- | |-- | ||
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| T/W Oct 3/4 | | T/W Oct 3/4 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F17): | + | | [[20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)| Examine sub-nuclear foci abundance to measure DNA damage]]<br>[[Media:M1D6_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17 M1D6 NLL marked.pdf| Prelab W/F]] |
− | | | + | | [[20.109(F17):Homework#Due_M1D6|Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
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| R/F Oct 5/6 | | R/F Oct 5/6 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109( | + | | [[20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)| Visualize and analyze data for sub-nuclear foci assay]]<br>[[Media:M1D7_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M1D7_JB_marked.pdf| Prelab W/F]] |
− | | [[20.109( | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M1D7|Homework due]] |
|-- | |-- | ||
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| <font color = 999999>Columbus day holiday</font color> | | <font color = 999999>Columbus day holiday</font color> | ||
− | | [[20.109(F17): M1 Data Summary| Data Summary draft due]] | + | | [[20.109(F17): M1 Data Summary| Data Summary draft due]] <font color = red>Wed, Oct 11 at 10 pm</font color = red> |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
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| 1 | | 1 | ||
| R/F Oct 12/13 | | R/F Oct 12/13 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D1 lecture slides.pdf| Lecture M2D1]] |
− | | [[20.109( | + | | [[20.109(F17):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:M2D1_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D1_Fa17_JB_withLigationCalculation.pdf| Prelab W/F]]<br> |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M2D1| Homework due]]<br>[[20.109(F17): M1 Mini-presentation| Mini-presentation due]] Sat, Oct 14 at 10 pm |
|-- | |-- | ||
| 2 | | 2 | ||
| 2 | | 2 | ||
− | | T/W Oct | + | | T/W Oct 17/18 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D2 lecture slides.pdf| Lecture M2D2]] |
− | | [[20.109( | + | | [[20.109(F17):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:M2D2_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D2_Fa17_JB_marked.pdf| Prelab W/F]]<br> |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M2D2|Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
− | | R/F Oct | + | | R/F Oct 19/20 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
− | | [[20.109( | + | | [[20.109(F17):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:M2D3_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D3_Fa17_JB_marked.pdf| Prelab W/F]] |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M2D3|Homework due]]<br>[[20.109(F17): M1 Data Summary| Data Summary revision due]] Sun, Oct 22 at 10 pm |
|-- | |-- | ||
| 2 | | 2 | ||
| 4 | | 4 | ||
− | | T/W Oct | + | | T/W Oct 24/25 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D4 lecture slides.pdf| Lecture M2D4]] |
− | | [[20.109( | + | | [[20.109(F17):Journal club I (Day4)| Journal club I]] |
− | | | + | | [http://be20109f17.blogspot.com/ Blog post due] Mon, Oct 23 at 10 pm |
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
− | | R/F Oct | + | | R/F Oct 26/27 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D5 lecture slides.pdf| Lecture M2D5]] |
− | | [[20.109( | + | | [[20.109(F17):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:Fa17_M2D5_EricLehnhardt_marked_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M2D5_JB_marked.pdf| Prelab W/F]] |
− | | [[20.109( | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D5|Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 6 | | 6 | ||
− | | T/W Nov | + | | T/W Oct/Nov 31/1 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D6 lecture slides.pdf| Lecture M2D6]] |
− | | [[20.109( | + | | [[20.109(F17):Journal club II (Day 6) | Journal club II]] |
− | | | + | | |
|-- | |-- | ||
| 2 | | 2 | ||
| 7 | | 7 | ||
− | | R/F Nov | + | | R/F Nov 2/3 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D7 lecture slides.pdf| Lecture M2D7]] |
− | | [[20.109( | + | | [[20.109(F17):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:M2D7_Prelab_TR_ECL_marked.pdf| Prelab T/R]]<br>[[Media:M2D7_Prelab_WF_JB_marked.pdf| Prelab W/F]]<br> |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M2D7|Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 8 | | 8 | ||
− | | T/W Nov | + | | T/W Nov 7/8 |
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media: | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa17 M2D8 lecture slides.pdf| Lecture M2D8]] |
− | | [[20.109( | + | | [[20.109(F17)::Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:M2D8_Fa17_LMM_1.pdf| Prelab T/R]]<br>[[Media:M2D8_Fa17_WF_JB.pdf| Prelab W/F]] |
− | | <font color=# | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M2D8|Homework due]] |
|-- | |-- | ||
| | | | ||
| | | | ||
− | | <font color = 999999>R/F Nov | + | | <font color = 999999>R/F Nov 9/10</font color> |
| | | | ||
| <font color = 999999>Veterans' day holiday</font color> | | <font color = 999999>Veterans' day holiday</font color> | ||
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| 3 | | 3 | ||
| 1 | | 1 | ||
− | | T/W Nov | + | | T/W Nov 14/15 |
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109( | + | | [[20.109(F17):Growth of phage materials (Day1)| Prepare phage materials]]<br>[[Media:F17_M3D1_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D1_WF_JB_marked.pdf| Prelab W/F]]<br> |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M3D1|Homework due]] <br> |
|-- | |-- | ||
| 3 | | 3 | ||
| 2 | | 2 | ||
− | | R/F Nov | + | | R/F Nov 16/17 |
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | [[20.109( | + | | [[20.109(F17):Purify active materials (Day2)| Purify active material for cathode construction]]<br>[[Media:F17_M3D2_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D2_JB_marked.pdf| Prelab W/F]]<br> |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M3D2|Homework due]]<br> |
|-- | |-- | ||
| | | | ||
| | | | ||
− | | T/W Nov | + | | T/W Nov 21/22 |
− | | | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:BATTERIES 20.109 Fall 2017.pdf| Lecture]] |
− | | <font color = # | + | | <font color = #31B404>'''Lecture, but no laboratory'''</font color> |
− | | | + | | [[20.109(F17): Research article| Research article due]] Mon, Nov 20 at 10 pm <br>[http://be20109f17.blogspot.com/ Blog post due] Tue, Nov 21 at 10 pm |
|-- | |-- | ||
| | | | ||
| | | | ||
− | | <font color = 999999>R/F Nov | + | | <font color = 999999>R/F Nov 23/24</font color> |
| | | | ||
| <font color = 999999>Thanksgiving holiday</font color> | | <font color = 999999>Thanksgiving holiday</font color> | ||
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| 3 | | 3 | ||
| 3 | | 3 | ||
− | | T/W Nov | + | | T/W Nov 28/29 |
− | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB] |
− | | [[20.109( | + | | [[20.109(F17):Cathode construction (Day3)| Construct cathode with active material]]<br>[[Media:F17_M3D3_ECL_marked.pdf| Prelab T/R]]<br>[[Media:F17_M3D3_JB_marked.pdf| Prelab W/F]]<br> |
− | | <font color=# | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D3| Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| 4 | | 4 | ||
− | | R/F Dec | + | | R/F Nov/Dec 30/1 |
− | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB] <br>[[Media:20.109 TEM Fall 2017.pdf| Lecture]] |
− | | [[20.109( | + | | [[20.109(F17):Visualize active material using TEM (Day4)| Visualize active material using TEM]]<br>[[Media:Fa17_M3D4_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M3D4_JB_marked.pdf| Prelab W/F]] |
− | | [[20.109( | + | | [[20.109(F17):Homework#Due_M3D4|Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| 5 | | 5 | ||
− | | T/W Dec | + | | T/W Dec 5/6 |
− | | [http://be.mit.edu/directory/angela-belcher AB] | + | | [http://be.mit.edu/directory/angela-belcher AB]<br>[[Media:Battery data 20.109 Fall 2017.pdf| Lecture]] |
− | | [[20.109( | + | | [[20.109(F17):Assemble and test batteries (Day5)| Assemble and test batteries]]<br>[[Media:Fa17_M3D5_LMM_1.pdf| Prelab T/R]]<br>[[Media:Fa17_M3D5_JB.pdf| Prelab W/F]] |
− | | <font color=# | + | | <font color=#0174DF>'''Laboratory quiz'''</font color><br>[[20.109(F17):Homework#Due_M3D5|Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| 6 | | 6 | ||
− | | R/F Dec | + | | R/F Dec 7/8 |
| | | | ||
− | | [[20.109( | + | | [[20.109(F17):Research proposal presentation| Research proposal presentations]] |
− | | [[20.109( | + | | [[20.109(F17):Research proposal presentation| Research proposal presentation slides due]] Thu/Fri, Dec 7/8 at 1 pm<br>[http://be20109f17.blogspot.com/ Blog post due] Fri, Dec 8 at 10 pm |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
Line 216: | Line 216: | ||
| | | | ||
| | | | ||
− | | T Dec | + | | T Dec 12 |
| | | | ||
− | | <font color = # | + | | <font color = #31B404>'''Feedback and celebratory lunch'''</font color> |
− | | [[20.109( | + | | [[20.109(F17): Biomaterials engineering mini-report | Biomaterials engineering mini-report due]] Mon, Dec 11 at 10 pm <br>[http://be20109f17.blogspot.com/ Blog post due] Tue, Dec 12 at 10pm |
|} | |} | ||
</div> | </div> |
Latest revision as of 20:44, 6 December 2017
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11-12 (16-220)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)