Difference between revisions of "Assignment 1 Overview: Transillumination microscopy"
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==Introduction== | ==Introduction== | ||
[[Image:20.309 130905 InstructorMicroscope1.png|300 px|thumb|right|20.309 microscope|Example 20.309 microscope.]] | [[Image:20.309 130905 InstructorMicroscope1.png|300 px|thumb|right|20.309 microscope|Example 20.309 microscope.]] | ||
− | Over the next few weeks, you will build an optical microscope using lenses, mirrors, filters, optical mounts, CMOS | + | Over the next few weeks, you will build an optical microscope using lenses, mirrors, filters, optical mounts, a CMOS camera, LEDs, and other components in the lab. The work is divided into 5 assignments. Each assignment requires some problem solving, some lab work, some analysis, lots of clear thinking, and an individually written answer sheet turned in on Stellar. All of the items you are expected to turn in are indicated by a pencil symbol in the lab manual. |
{{Template:Assignment Turn In|message =This symbol means that you have to turn something in.}} | {{Template:Assignment Turn In|message =This symbol means that you have to turn something in.}} | ||
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Part 1: | Part 1: | ||
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# Answers to the pre-lab questions [[Assignment 1, Part 1: Pre-lab questions|listed at the bottom of the Part 1 page]]. | # Answers to the pre-lab questions [[Assignment 1, Part 1: Pre-lab questions|listed at the bottom of the Part 1 page]]. | ||
Part 2: | Part 2: | ||
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# Display an example image of the ruler at each magnification, and | # Display an example image of the ruler at each magnification, and | ||
− | # Make a table of displaying the nominal magnification (i.e. the printed number on the objective), the expected magnification (based on the 125 mm tube lens), the object height, the image height, the actual (i.e. measured) magnification and the FOV (see example). Don't forget to include appropriate units. Report the length and width of the FOV (in distance units), not its area (in distance units squared) | + | # Make a table of displaying the nominal magnification (i.e. the printed number on the objective), the expected magnification (based on the 125 mm tube lens), the object height, the image height, the actual (i.e. measured) magnification and the FOV (see example). Don't forget to include appropriate units. Report the length and width of the FOV (in distance units), not its area (in distance units squared) |
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# Display an example image of each bead size. | # Display an example image of each bead size. | ||
# Report the average size and uncertainty of the spheres in each sample, (be sure to include the number of samples measured). | # Report the average size and uncertainty of the spheres in each sample, (be sure to include the number of samples measured). |
Latest revision as of 17:05, 11 February 2020
Introduction
Over the next few weeks, you will build an optical microscope using lenses, mirrors, filters, optical mounts, a CMOS camera, LEDs, and other components in the lab. The work is divided into 5 assignments. Each assignment requires some problem solving, some lab work, some analysis, lots of clear thinking, and an individually written answer sheet turned in on Stellar. All of the items you are expected to turn in are indicated by a pencil symbol in the lab manual.
This symbol means that you have to turn something in. |
Background reading and resources
You will work with log-log plots in this assignment and future ones. These seem to confuse everybody. Read this page to remind yourself how log-log plots work.
Several microscope manufacturers maintain educational websites, including Nikon's MicroscopyU, Olympus' Microscopy Primer, and the Zeiss online microscopy campus. The content on these sites ranges from basic concepts like Snell's law and Resolution to advanced techniques like super resolution imaging.
Assignment details
This assignment has 4 parts:
- Part 1: Learn about optics and answer a few questions before you start your lab work;
- Part 2: Some warm-up lab exercises;
- Part 3: You will build a microscope; and finally you will
- Part 4: Measure its magnification and the size of some small beads.
You will add fluorescence capability in the next part of the lab.
Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment1.pdf. Here is a checklist of all things you have to turn in:
Make sure to include answers to all the following questions: Part 1:
Part 2:
Parts 3 and 4:
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- Overview
- Part 1: Pre-lab questions
- Part 2: Optics bootcamp
- Part 3: Build a microscope
- Part 4: Measure stuff
Back to 20.309 Main Page
References