Difference between revisions of "20.109(S19): Spring 2019 schedule"
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Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
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| Line 19: | Line 19: | ||
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| − | | T/W Feb | + | | T/W Feb 5/6 |
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109(S18):Laboratory tour | Orientation and laboratory tour]]<br> | + | | [[20.109(S18):Laboratory tour | Orientation and laboratory tour]]<br> |
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|-- | |-- | ||
| Line 28: | Line 28: | ||
| 1 | | 1 | ||
| 1 | | 1 | ||
| − | | R/F Feb | + | | R/F Feb 7/8 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br> |
| − | | <font color= #3498db>'''Orientation quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Orientation quiz'''</font color> <br> [[20.109(S19):Homework#Due_M1D1| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 2 | | 2 | ||
| − | | T/W Feb | + | | T/W Feb 12/13 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Analyze small microarray data and induce protein expression (Day2) |Complete small microarray analysis and induce protein expression]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M1D2| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 3 | | 3 | ||
| − | | R/F Feb | + | | R/F Feb 14/15 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Purify protein for secondary assays(Day3) |Purify protein for secondary assays]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M1D3| Homework due]] |
|-- | |-- | ||
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| Line 56: | Line 56: | ||
| 1 | | 1 | ||
| 4 | | 4 | ||
| − | | R/F Feb | + | | R/F Feb 21/22 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br> |
| − | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M1D4| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 5 | | 5 | ||
| − | | T/W Feb | + | | T/W Feb 26/27 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M1D5| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 6 | | 6 | ||
| − | | R/F Mar | + | | R/F Feb/Mar 28/1 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M1D6| Homework due]] |
|-- | |-- | ||
| 1 | | 1 | ||
| 7 | | 7 | ||
| − | | T/W Mar | + | | T/W Mar 5/6 |
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Complete data analysis (Day7) |Complete data analysis]]<br> |
| − | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M1D7| Homework due]] |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 86: | Line 86: | ||
| 2 | | 2 | ||
| 1 | | 1 | ||
| − | | R/F Mar | + | | R/F Mar 7/8 |
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1)| Practice tissue culture techniques and prepare cells for RNA purification]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M2D1| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 2 | | 2 | ||
| − | | T/W Mar | + | | T/W Mar 12/13 |
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
| − | | [[20.109( | + | | [[20.109(S19):Induce DNA damage and apply drug treatments for RNA purification (Day2)| Induce DNA damage and apply drug treatments for RNA purification]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M2D2| Homework due]]<br> [[20.109(S19): M1 Data Summary| <font color=#FF0000>Data summary draft due</font color>]] Mon, Mar 11 at 10 pm<br> |
|-- | |-- | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
| − | | R/F Mar 15 | + | | R/F Mar 14/15 |
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF] <br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF] <br> |
| − | | [[20.109( | + | | [[20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)| Purify RNA and practice RNA-seq data analysis methods]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M2D3| Homework due]] <br> [[20.109(S19):Mini-presentation| <font color=#FF0000>Mini-presentation due</font color>]] Sat, Mar 16 at 10 pm<br>[http Blog post due] Sun, Mar 17 at 10 pm <br> |
|-- | |-- | ||
| 2 | | 2 | ||
| 4 | | 4 | ||
| − | | T/W Mar | + | | T/W Mar 19/20 |
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)| Analyze RNA-seq data and prepare for quantitative PCR experiment]]<br> |
| − | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M2D4| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| − | | R/F Mar | + | | R/F Mar 21/22 |
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Investigate RNA-seq data using public databases (Day5) | Investigate RNA-seq data using public databases]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M2D5| Homework due]] |
|-- | |-- | ||
| | | | ||
| | | | ||
| − | | T/W Mar | + | | T/W Mar 26/27 |
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| <font color = #0404B4>'''Spring vacation'''</font color> | | <font color = #0404B4>'''Spring vacation'''</font color> | ||
| − | | [[20.109( | + | | [[20.109(S19): M1 Data Summary| <font color=#FF0000>Data summary revision due</font color>]] Mon, Mar 25 at 10 pm |
|-- | |-- | ||
| | | | ||
| | | | ||
| − | | R/F Mar | + | | R/F Mar 28/29 |
| | | | ||
| <font color = #0404B4>'''Spring vacation'''</font color> | | <font color = #0404B4>'''Spring vacation'''</font color> | ||
| Line 135: | Line 135: | ||
| 2 | | 2 | ||
| 6 | | 6 | ||
| − | | T/W Apr | + | | T/W Apr 2/3 |
| Comm Lab<br> | | Comm Lab<br> | ||
| − | | [[20.109( | + | | [[20.109(S19):Journal club presentations (Day6 and 7)| Journal club I presentations]]<br> |
| | | | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 7 | | 7 | ||
| − | | R/F Apr | + | | R/F Apr 4/5 |
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Journal club presentations (Day6 and 7)| Journal club II presentations]]<br> |
| − | | [ | + | | [http Blog post due] Sat, Apr 7 at 10 pm <br> |
|-- | |-- | ||
| 2 | | 2 | ||
| 8 | | 8 | ||
| − | | T/W Apr | + | | T/W Apr 9/10 |
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8)| Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M2D8| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| 9 | | 9 | ||
| − | | R/F Apr | + | | R/F Apr 11/12 |
| − | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Complete cell viability assay (Day9)| Complete cell viability assay]]<br> |
| − | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M2D9| Homework due]] <br> |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
| Line 172: | Line 172: | ||
| 3 | | 3 | ||
| 1 | | 1 | ||
| − | | R/F Apr | + | | R/F Apr 18/19 |
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| − | | [[20.109( | + | | [[20.109(S19):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M3D1| Homework due]] <br> [[20.109(S19): Research article| <font color=#FF0000>Research article due</font color>]] Sat, Apr 20 at 10 pm <br> [http Blog post due] Sun, Apr 21 at 10 pm |
|-- | |-- | ||
| 3 | | 3 | ||
| 2 | | 2 | ||
| − | | T/W Apr | + | | T/W Apr 23/24 |
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| − | | [[20.109( | + | | [[20.109(S19):Purify phage-based active (cathode) material (Day2) | Purify phage-based active (cathode) material]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M3D2| Homework due]] |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|-- | |-- | ||
| 3 | | 3 | ||
| | | | ||
| − | | | + | | R/F Apr 25/26 |
| − | | | + | | |
| <font color = #0404B4>'''Lecture, but no laboratory'''</font color> | | <font color = #0404B4>'''Lecture, but no laboratory'''</font color> | ||
| | | | ||
| + | |-- | ||
| + | | 3 | ||
| + | | 3 | ||
| + | | T/W Apr/May 30/1 | ||
| + | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
| + | | [[20.109(S19):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br> | ||
| + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M3D3| Homework due]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 4 | | 4 | ||
| − | | R/F May | + | | R/F May 2/3 |
| − | | [http://be.mit.edu/directory/angela-belcher AB]<br> | + | | [http://be.mit.edu/directory/angela-belcher AB]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using transmission electron microscopy]]<br> |
| − | | [[20.109( | + | | [[20.109(S19):Homework#Due_M3D4| Homework due]] |
|-- | |-- | ||
| 3 | | 3 | ||
| 5 | | 5 | ||
| − | | T/W May | + | | T/W May 7/8 |
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| − | | [[20.109( | + | | [[20.109(S19):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br> |
| − | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109( | + | | <font color= #3498db>'''Laboratory quiz'''</font color> <br> [[20.109(S19):Homework#Due_M3D5| Homework due]] |
|-- | |-- | ||
| | | | ||
| | | | ||
| − | | R/F May | + | | R/F May 9/10 |
| | | | ||
| − | | [[20.109( | + | | [[20.109(S19):Research proposal presentation | Research proposal presentations]] |
| − | | [ | + | | [http Blog post due] Sat, May 11 at 10 pm |
|-- | |-- | ||
| | | | ||
| | | | ||
| − | | T May | + | | T May 14 |
| | | | ||
| <font color = #0404B4>'''Feedback and celebratory lunch'''</font color> | | <font color = #0404B4>'''Feedback and celebratory lunch'''</font color> | ||
| − | | [[20.109( | + | | [[20.109(S19): Biomaterials engineering mini-report | <font color=#FF0000>Mini-report due</font color>]] Mon, May 13 at 10 pm <br> |
|} | |} | ||
</div> | </div> | ||
Revision as of 20:01, 8 January 2019
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (4-149)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
