Difference between revisions of "DNA Melting: DNA Sequences"
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− | For double-stranded DNA solutions, one of the 20bp, 30bp, or 40bp sequences is mixed in equal concentration with its respective complement, e.g., 30bp and 30bp-comp, in a desired saline solution. For 20bp mismatch solutions, the 20bp sequence is mixed in equal concentration with one of the mismatch sequences. | + | For double-stranded DNA solutions, one of the 20bp, 30bp, or 40bp sequences is mixed in equal concentration with its respective complement, ''e.g.'', 30bp and 30bp-comp, in a desired saline solution. For 20bp mismatch solutions, the 20bp sequence is mixed in equal concentration with one of the mismatch sequences. Unless otherwise stated, the DNA concentration is 30 μM, and [KCl] = 100 mM. |
+ | |||
+ | Sample naming key: | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |- | ||
+ | ! Name !! Sample !! Salt concentration !! number of mismatches | ||
+ | |- | ||
+ | |A || 20bp|| 100mM NaCl || none | ||
+ | |- | ||
+ | |B ||20bp||200mM NaCl || none | ||
+ | |- | ||
+ | |C||20bp|| 50mM NaCl|| none | ||
+ | |- | ||
+ | |D||30bp|| 100mM NaCl|| none | ||
+ | |- | ||
+ | |E||40bp|| 100mM NaCl|| none | ||
+ | |- | ||
+ | |F||20bp-single mismatch|| 100mM NaCl|| one | ||
+ | |- | ||
+ | |G||20bp-double mismatch|| 100mM NaCl|| two | ||
+ | |} |
Latest revision as of 00:03, 10 November 2017
Single-stranded DNA sequences:
Name | Sequence | MW | GC % |
---|---|---|---|
20bp | ATC AAG CAG CCA TCG AAA CT | 6079 | 45 |
20bp-comp | AGT TTC GAT GGC TGC TTG AT | 6154 | 45 |
20bp-single mismatch | AGT TTC GAT TGC TGC TTG AT | 6129 | 40 |
20bp-double mismatch | AGT TTC GAT TGA TGC TTG AT | 6153 | 35 |
20bp-complete mismatch | CAC CGA CGT ACT ATT CCT AA | 6021 | 45 |
30bp | ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA | 9168 | 43.3 |
30bp-comp | TGT AAG CCT AAG TTT CGA TGG CTG CTT GAT | 9243 | 43.3 |
40bp | ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA ACC AGT GAC T | 12242 | 45 |
40bp-comp | AGT CAC TGG TTG TAA GCC TAA GTT TCG ATG GCT GCT TGA T | 12348 | 45 |
For double-stranded DNA solutions, one of the 20bp, 30bp, or 40bp sequences is mixed in equal concentration with its respective complement, e.g., 30bp and 30bp-comp, in a desired saline solution. For 20bp mismatch solutions, the 20bp sequence is mixed in equal concentration with one of the mismatch sequences. Unless otherwise stated, the DNA concentration is 30 μM, and [KCl] = 100 mM.
Sample naming key:
Name | Sample | Salt concentration | number of mismatches |
---|---|---|---|
A | 20bp | 100mM NaCl | none |
B | 20bp | 200mM NaCl | none |
C | 20bp | 50mM NaCl | none |
D | 30bp | 100mM NaCl | none |
E | 40bp | 100mM NaCl | none |
F | 20bp-single mismatch | 100mM NaCl | one |
G | 20bp-double mismatch | 100mM NaCl | two |