Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"
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===OD562 absorbance measurements for protein concentration calculations=== | ===OD562 absorbance measurements for protein concentration calculations=== | ||
| − | During the 1 hour incubation of | + | During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data [[Media:SampleData_microBCA.xlsx| '''here'''.]] You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical [[Media: How to use a protein standard curve.pdf | bulletin]] helpful. |
====T/R red team==== | ====T/R red team==== | ||
{| border="1" | {| border="1" | ||
Revision as of 13:34, 26 February 2016
OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.
T/R red team
| name | OD562 |
| standards | |
| A | 3.02 |
| B | 0.798 |
| C | 0.444 |
| D | 0.245 |
| E | 0.141 |
| F | 0.073 |
| G | 0.054 |
| I | 0.034 |
| inverse pericam (IPC) | |
| wild-type | 0.177 |
| mutant | 0.201 |
